This quantitative PCR (qPCR)-based kit—part of the resDNASEQ® E. coli
Residual DNA Quantitation System—enables highly sensitive quantitation of host cell DNA from E. coli
expression systems, typically in less than 4 hours.
The kit is part of an integrated system that includes sample preparation, TaqMan® Assay and master mix, standard DNA, instrument, and software. The system overcomes the limitations of traditional methods by combining high-recovery PrepSEQ® sample preparation and TaqMan® Assay-based quantitation of residual DNA.
• Highly sensitive quantitation using proven TaqMan® real-time qPCR technology (Figure 1)
• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices (Table 1)
• Consistent performance across the expected range of DNA fragment sizes (Figure 2)
• Integrated system from sample to results with sample preparation, master mix, TaqMan® primer/probe mix, and genomic DNA standardRapid, Sensitive Detection of E.Coli DNA
The resDNASEQ® E. coli
Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA fromE. coli
, an expression system commonly used for the production of recombinant proteins. Reliable and rapid, the system enables sensitive (LOQ = 15 pg DNA/ml test sample, Figure 1) and specific (Figures 2 and 3) quantitation ofE. coli
DNA, typically in less than 4 hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to bulk drug substances—whether a sample contains high molecular weight or sheared DNA (Figure 3).Table 1. DNA recovery using manual PrepSEQ® sample preparation protocol.
Assay performance data using 10 pg E. coli
genomic DNA spike per sample, 3 analysts, and 9 test samples.
| || 50 mg/ml protein sample|| |
| || Mean Recovery || Mean %CV |
| E. coli ||83%||5.04%|
For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.