Applied Biosystems™

TaqMan™ Multiplex Master Mix

Catalog number:  4461881

 Related applications:

Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

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Applied Biosystems® TaqMan® Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (<40 minutes) with excellent concordance of results in singleplex and multiplex reactions. TaqMan® Multiplex Master Mix contains AmpliTaq® Fast DNA PolymeraseUracil-N Glycosylase (UNG), dNTPs with dUTP, MUSTANG PURPLE® dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration.

Features of TaqMan® Multiplex Master Mix include:

• Optimized for multiplexing—validated for quadplex gene expression and genotyping assays
• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity
• Reduced run times on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
• Seamless integration into your experiments—validated with Applied Biosystems® TaqMan® assays and TaqMan® QSY® probes for gene expression and genotyping experiments
• Carry-over contamination control—contains heat-labile UDG
• Broad instrument compatibility

Optimized for Multiplexing
Confidence is paramount when it comes to your results. TaqMan® Multiplex Master Mix is designed to deliver accurate results for multplex reactions and ensure excellent concordance of results in both singleplex and multiplex reactions.

Best-in-class Performance
TaqMan® Multiplex Master Mix is designed for performance equal to or better than other multiplex master mixes on the market. TaqMan® Multiplex Master Mix has been benchmarked against the leading suppliers' multiplex master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity.

Reduced Run Times on Standard Instrumentation
TaqMan® Multiplex Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides.

Carry-over Contamination Control
TaqMan® Multiplex Master Mix contains heat-labile uracil-DNA glycosylase (UDG) for worry-free carryover contamination control. The dUTP in the mix ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs. A UDG incubation step before PCR cycling destroys any contaminating dU-containing product from previous reactions. UDG is then inactivated by the high temperatures during normal PCR cycling, thereby allowing the amplification of genuine target sequences.

Instrument Compatibility
TaqMan® Multiplex Master Mix can be used in fast cycling mode on both standard and fast qPCR instruments, such as: 7500 Real-Time PCR System, 7500 Fast Real-Time PCR System, ViiA™ 7 Real-Time PCR System, and QuantStudio™ 6 Flex, 7 Flex, and 12K Flex Real-Time PCR systems.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System
Reaction Speed: Fast
PCR Method: Multiplex PCR
Sample Type (General): DNA
Number of Reactions: 100
Polymerase: AmpliTaq® Fast DNA Polymerase
Product Line: TaqMan™
Product Size: 1 mL, 1 x 1 mL

Contents & storage

Contains 1 tube (1 mL) of 2X TaqMan® Multiplex Master Mix sufficient for 100 reactions.

TaqMan® Multiplex Master Mix contains AmpliTaq® Fast DNA Polymerase, uracil-N glycosylase (UNG), dNTPs (with dUTP), MUSTANG PURPLE® dye (passive reference), and optimized buffer.

Store in refrigerator (2–8°C).


Manuals & protocols