Applied Biosystems™

SOLiD™ FWD ECC Module, Double

Catalog number:  4463032

 Related applications: SOLiD® Next-Generation Sequencing

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The SOLiD™ FWD ECC Module is an optional kit that is used to further enhance the sequencer accuracy on the 5500⁄5500xl Genetic Analysis Systems. The kit is based on the SOLiD™ Exact Call Chemistry (ECC), which generates data encoded distinctly from 2-base encoded (2BE) data. The SOLiD™ FWD ECC Module provides orthogonal proofreading to yield highly accurate sequences (up to 99.99%), and can also generate reference-free base sequence. Double size contains enough reagents for two FlowChips.

The SOLiD™ FWD ECC Module is used for fragment and mate-paired ECC sequencing runs, and for ligation sequencing in the forward direction using an additional primer. The kit features primers, enzyme, and ECC probes used in an optional ligation sequencing step, which follows "classic" ligation sequencing with 2BE probes.

The SOLiD™ FWD ECC Module requires the SOLiD™ Run Cycle Buffers, SOLiD™ FWD Buffer, SOLiD™ Buffer D, SOLiD™ FWD SR Kit, and at least one of the following: SOLiD™ FWD1 SP Kit, SOLiD™ FWD2 SP Kit, or SOLiD™ Small RNA SP Kit.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): 5500 Genetic Analyzer, 5500xl Genetic Analyzer
Recommended Applications: Small Genome Sequencing, Targeted Resequencing
Downstream Application: Next-Generation Sequencing
Library Type: Fragment Library, Mate-Paired Library
Product Line: SOLiD™
Product Size: 1 kit
Sequencing Type: Genome & DNA Sequencing
Starting Material: DNA
Workflow Step: Sequencing
Shipping Condition: Dry Ice

Contents & storage

The kit contains sufficient reagents to run 120 to 180 cycles, or up to 15 cycles per lane. Store the SOLiD™ FWD ECC Module at -15°C to -25°C until ready for use.


Manuals & protocols