Library Builder™ Fragment Core Kit for 5500 Genetic Analysis Systems

Catalog number: 4463763

Applied Biosystems™  Related applications: SOLiD® Next-Generation Sequencing

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Description

The Library Builder™ Fragment Core Kit for 5500 Series Genetic Analysis Systems is the reagent component of the AB Library Builder™ System. This ready-to-use reagent cartridge simplifies next-generation sequencing by providing a semi-automated solution for DNA fragment library preparation for the 5500 Series Genetic Analysis Systems.

The kit requires minimal set-up. The reagent cartridges contain the necessary components (enzymes, buffers) for fragment library construction. When used with SOLiD® Fragment Library Oligos, barcodes, and Agilent AMPure® Beads, the kit allows for the creation of up to 13 DNA fragment libraries.
• Automated—reduces hands-on time by 50%
• Scalable—prepares up to 13 libraries per run, up to 26 libraries per day
• Integrated solution optimized for library preparation for the 5500 Series Genetic Analysis Systems

Automated Workflow
The Library Builder™ Fragment Core Kit for 5500 Series Genetic Analysis Systems, when used with the AB Library Builder™ System, automates the most labor-intensive parts of DNA fragment library preparation, reducing hands-on time by at least 50%. The AB Library Builder™ System provides full walk-away automation of the post-shearing and pre–nick translation portion of the DNA fragment library workflow for exome and targeted resequencing on either the 5500 Genetic Analyzer or 5500xl Genetic Analyzer.

Scalable Throughput
The Library Builder™ Fragment Core Kit for 5500 Series Genetic Analysis Systems contains enough reagents and materials to process up to 13 samples at a time.

Integrated Solution
The Library Builder™ Fragment Core Kit for 5500 Series Genetic Analysis Systems along with the AB Library Builder™ System is an integrated solution comprising a predefined software protocol, plug-and-play cartridge format, reagents, and instrument—all optimized and validated for DNA purification and DNA fragment library preparation with the 5500 Series Genetic Analysis Systems.

Simplified and Cost-effective DNA Fragment Libraries
The Library Builder™ Fragment Core Kit for 5500 Series Genetic Analysis Systems and AB Library Builder™ System enable higher DNA fragment library throughput while reducing the hands-on time needed to prepare libraries. We are committed to simplifying the sequencing workflow.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): 5500xl Genetic Analyzer, 5500 Genetic Analyzer
Library Type: Fragment Library
Workflow Step: Library Generation, Library Barcoding
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Product Size: 1 kit
Final Product: DNA
Downstream Application: Next-Generation Sequencing

Contents & storage

The Reagents Module (store at -20°C) contains:
• 13 cartridges containing ready-to-use reagents
• 13 tubes of 5x Reaction Buffer
• 1 tube of Shear Buffer; 5 tubes of Platinum® PCR Amplification mix
• 1 tube each of Library PCR Primer 1 and Library PCR Primer 2

The Plastics Module (store at room temperature) contains sample and elution tubes, tips, and tip holders.

The materials in this kit are sufficient to prepare up to 13 standard or express fragment libraries.

Documents

Manuals & protocols