5500 SOLiD™ Mate-Paired Library Kit

Catalog number: 4464418

Applied Biosystems™  Related applications: SOLiD® Next-Generation Sequencing

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Description

Structural variations are present throughout the genome and are associated with various diseases. Mate-paired libraries, in conjunction with 5500 Series Genetic Analysis Systems, provide a valuable method for detecting genomic rearrangements such as insertions, deletions, duplications, and translocations. The 5500 SOLiD™ Mate-Paired Library Kit provides a fully optimized set of enzymes and reagents needed for construction of 12 Mate-Paired libraries from as little as 1 µg of DNA input.*

Benefits of the 5500 SOLiD™ Mate-Paired Library Kit:
• A convenient and simplified workflow—includes all reagents, columns, and gels for the construction of 12 libraries
• Optimized and platform flexible protocol for accurate analysis with either the 5500 Series Genetic Analysis Systems or SOLiD™ 4 System.
• Offers a process friendly protocol with low DNA input requirement (1–5 µg) and 3-to 10-fold yield improvement over the previous kit.
• Provides a lower cost per Mate-Paired library compared to the previous kit.

5500 SOLiD™ mate-paired sequencing can generate sequencing reads with a distance ranging from a few hundreds to tens of hundreds of base pairs from as little as 1 µg of input DNA. It provides critical information for shotgun based whole genome sequencing and structural variation study in a massively parallel fashion. The 5500 SOLiD™ Mate-Paired Library Kit uses a new intramolecular circularization (NIC) method and optimized workflow which removes most column-based purification steps. This new LMP protocol improves the library yield 3-to-10 fold depending on the insert size and input amount. Mate-paired sequencing has eliminated adaptor dimer carry-over in the final library and further decreased false mate-pairing rate. This approach not only produces most balanced tag sizes on each end, but is also very scalable on tag length to accommodate consistent increase on sequencing read length.

*We recommend using 1 µg of input for low coverage or less complex genomes, and 5 µg for higher coverage or more complex genomes.

Note: AMPure® Beads are not included and must be purchased directly from Beckman Coulter.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): 5500xl Genetic Analyzer, 5500 Genetic Analyzer, SOLiD™ 4 System
Library Type: Mate-Paired Library
Workflow Step: Library Generation
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Product Size: 1 kit
Final Product: Mate-Paired Library
Downstream Application: Next-Generation Sequencing
Recommended Applications: Small Genome Sequencing, Targeted Resequencing

Contents & storage

Each kit contains 7 modules (enough reagents for 12 libraries):
• Enzymes (-20°C)
• Dynabeads and buffers (4°C)
• PCR Amplification mix (-20°)
• P1-T & P2-T adaptors (-20°)
• Library PCR Primers 1 and 2, MPR & MPL adaptors (-20°)
• Purification columns (room temperature)
• Gel extraction kits (room temperature)

Documents

Manuals & protocols