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PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating

Catalog number: 4464654

Applied Biosystems™  Related applications: Food & Beverage

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The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating was developed for the AFNOR-approved MicroSEQ® Listeria species and Listeria monocytogenes Real-Time PCR detection workflows. A combined spin and bead beating protocol efficiently lyses Listeria from a 750 µL aliquot of enriched food samples (those with little-to-no fat and lean meats) and simple environmental samples (swabs and sponges). The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating is designed to meet the needs of lower throughput food laboratories.

NOTE: Difficult samples (e.g., samples containing high fat or otherwise prone to PCR inhibition) should be processed using the PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating.

• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput (up to 100 samples a day)
• Inactivates PCR inhibitors and produces high quality DNA for downstream detection
• Uses a simple spin protocol

Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types.

Designed for Laboratories that Prepare up to 100 Samples a Day
Spin column-based preparation enables small batch sizes to be managed while requiring only simple benchtop devices to process, thereby limiting the capital expenditure associated with much higher throughput automation devices.

Removes PCR Inhibitors and Produces High Quality DNA
The PrepSEQ® Rapid Spin Sample Prep Kit - Bead Beating offers a simple, cost-effective way to prepare Listeria DNA from enriched cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube, where they are then lysed via a zirconium bead beating step.
For Testing of Food and Environmental Samples Only.


Sample Type (General): Food & Environmental Samples
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible
Product Size: 100 preps

Contents & storage

Store lysis buffer in refrigerator (2–8°C). DO NOT FREEZE.


Manuals & protocols