Protein Thermal Shift™ Software v1.3

Catalog number: 4466038

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | Target & Lead Identification & Validation

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The Protein Thermal Shift™ Software v1.3 was developed to analyze protein melt fluorescent readings directly from Applied Biosystems® real-time PCR instrument files. Different proteins will have different Protein Thermal Shift™ profiles, each with a unique protein melt curve shape, slope, signal-to-noise ratio, and temperature melt range (see figure at right for an example). The Protein Thermal Shift™ Software generates one or multiple melting temperature values (Tm) from these curves by two methods: the Boltzmann-derived Tm and the Derivative Curve-determined Tm, to serve as points of comparison between the curves and represent the relative thermal stability of the protein under different testing conditions.

>> Download a trial version (Registration required)
>> Download the software (License required)

Two Methods for Generating Tm Value
The Boltzmann method fits the data within an automatic (or manually) identified melt region to the two-state Boltzmann model to generate the Tm. Typically, for proteins with a single melt domain, the Boltzmann method is used. However, for proteins with multiple melt domains, the Derivative Curve method can be used. The Derivative Curve method uses a numerically-computed second derivative of the raw data to estimate the temperatures where up to six peaks (local maxima) may occur in the derivative profile. An empirically-derived threshold on the signal-to-noise ratio is used to determine which local maxima will be detected.

Compatible with our Real-Time PCR Systems
The Protein Thermal Shift™ analysis software is compatible with run files generated from these real-time PCR systems: QuantStudio™ 3, 5, 6, 7, and 12K Flex, StepOne™, StepOnePlus™, 7500, 7500 Fast, and ViiA™7.

Prepare Your Samples Using our Protein Thermal Shift™ Kits
Prepare your samples for analysis using our Protein Thermal Shift™ Starter Kit or Protein Thermal Shift™ Dye Kit (available separately). These kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.


For Use With (Equipment): 7500 System, 7500 Fast System, StepOne™, StepOnePlus™, ViiA™ 7 System
License Type: Static
Product Size: 1 license
Operating System: Windows 7 (Service Pack 2)
Software Category: Software


Manuals & protocols