PrepSEQ® Express Nucleic Acid Extraction Kit

Catalog number: 4466351

Applied Biosystems™  Related applications: Contaminant & Impurity QC Testing

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The PrepSEQ® Express Nucleic Acid Extraction Kit contains pre-filled reagents and plastic tubes and tips to perform hands-free extraction of purified nucleic acid from 52 samples. The kit is designed for use on the AutoMate Express™ Nucleic Acid Extraction System, which is an easy-to-use benchtop instrument that enables hands-free automation of the nucleic acid extraction process for contaminant and impurity testing. The cartridges in this kit utilize the established PrepSEQ® nucleic acid extraction chemistry for quantitative recovery of DNA and RNA from diverse samples types, including cell culture, raw material (i.e., serum), and in-process downstream purification samples such as column purification or bulk drug substance (BDS) samples.

Key Product Features:

• Time-saving, cost-effective DNA extraction for mycoplasma, MMV, vesivirus, and residual DNA quantitation
• Quantitative and reproducible recovery of DNA from complex samples
• Out-of-the-box automation when used with the AutoMate Express™ Nucleic Acid Extraction System
• Closed system minimizes the risk of contamination
• Ideal for in-process testing of contaminants and impurities

Three-in-One Extraction of Cell Culture Contaminants
The PrepSEQ® Express™ kit can be used to perform nucleic acid extraction of the common contaminants for mammalian cell culture manufacturing, i.e., mycoplasmas, MMV, and vesiviruses (Figure 1). Flexible protocols are available to isolate DNA and RNA from a single organism, or a 3-in-1 surveillance panel protocol can simultaneously extract mycoplasma, MMV, and vesivirus nucleic acid from a single sample.

Quantitative Recovery for Residual DNA Applications
The PrepSEQ® Express™ kit is also designed for highly efficient recovery of host cell residual DNA from complex samples typically recovered during biopharmaceutical purification processes. When used together with the AutoMate Express™ system, the PrepSEQ® Express™ kit facilitates quantitative and consistent recovery of DNA from challenging matrices, including samples that have high protein concentration, low pH, or high salt (Figure 2).

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Biopharmaceutical Production Samples
High Throughput Compatibility: Automated Protocols
Downstream Application: Real-Time Quantitative PCR (qPCR)
Product Size: 1 kit
Final Product: Total RNA, Genomic DNA
Purification Time: ≤ 1 hr
Number of Reactions: 52 Isolations
Isolation Technology: Magnetic Bead
Sample Type (Specific): Cell Cultures, Bulk Drug Substance Sample, Biopharmaceutical Purification Sample
For Use With (Equipment): 7500 Fast System, AutoMate Express™ Nucleic Acid Extraction System
Shipping Condition: Room Temperature

Contents & storage

Contains pre-filled reagent cartridges and plastics to perform extraction of DNA and RNA.

Store at room temperature.


Manuals & protocols

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