TaqMan® Mutation Detection IPC Reagent Kit

Catalog number: 4467538

Applied Biosystems™  Related applications: Genotyping & Genomic Profiling | Oncology & Genetic Disease Research | Real Time PCR (qPCR)

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Description

The TaqMan® Mutation Detection IPC Reagent Kit is a set of optional internal positive control reagents that can be duplexed with any TaqMan® Mutation Detection assay to provide a positive PCR control result. The IPC reagents can distinguish a mutation target negative result from a PCR failure result. The TaqMan® Mutation Detection IPC Reagent Kit is preoptimized for use with the TaqMan® Mutation Detection Assays. The IPC reagents can be duplexed with any TaqMan® Mutation Detection Assay and gDNA sample, without compromising the mutation detection assay reaction efficiency. This feature allows the mutation detection assays to detect low copies of mutated DNA in the presence of a robust PCR-positive signal from the IPC reagents.

Product Overview

The TaqMan® Mutation Detection IPC Reagent Kit includes:
50X Exogenous IPC template DNA (200 µL)
A synthetic template that contains the exogenous, non-human DNA target for the assay

10X Exogenous IPC Mix (1 mL)
A primer-limited TaqMan® assay designed to amplify an exogenous, non-human DNA target; the mix includes:
• Two PCR primers
• A TAMRA™ probe (VIC® dye-labeled)

Store at –15°C to –25° C, protected from light. Minimize freeze-thaw cycles.

Instrument Compatibility
TaqMan® Mutation Detection IPC Reagent Kit is compatible with the Applied Biosystems® ViiA™ 7, 7900HT, 7500, 7500 Fast, and StepOnePlus™ Real-Time PCR Systems.

Sample Type Compatibility
The kit can be used with FFPE samples, cell lines, and fresh frozen samples.

Specifications

Control Template: non-human DNA
Control Type: Positive Control
Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System, 7500 System, StepOnePlus™, ViiA™ 7 System
Includes: Control Primer-Probe Set(s) & Template(s)
Label or Dye: TAMRA™, VIC®
Product Line: Applied Biosystems™, TaqMan™
Product Size: 500 reactions
Sample Type (General): Cells, DNA, FFPE & Fixed Samples
Species: Non-Human
Target Gene: exogeneous, non-human DNA target
Shipping Condition: Dry Ice

Contents & storage

Store at –15°C to –25° C, protected from light. Minimize freeze-thaw cycles.

Documents

Manuals & protocols