Applied Biosystems™

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit

Catalog number: 4467831
Have Questions?
Applied Biosystems™

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit

Catalog number: 4467831
Have Questions?
Catalog Number
Unit Size
200 reactions
Price (USD)
Catalog Number
Unit Size
1000 reactions
Price (USD)
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4467831200 reactions
44085801000 reactions
Product Overview
Citations & References
Additional Information
The AmpFLSTR Identifiler Direct PCR Amplification Kit enables direct amplification of the 16 loci included in the Identifiler Kit, eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Blood or buccal samples on FTA Cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector and other non-FTA substrates can be processed without the need for heat incubation or additional workflow steps using the Applied Biosystems Prep-n-Go Buffer.

Key Features of the AmpFLSTR Identifiler Direct PCR Amplification Kit:

• Enables the direct amplification of blood and buccal samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction/purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler Direct kit uses the same dye set, allelic ladder and primer sequences as the standard Identifiler kit, which:
• Maximizes concordance with STR profiles generated with other AmpFLSTR kits including the Identifiler, Identifiler Plus, Profiler Plus, Profiler Plus ID, SGM Plus and COfiler Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

Sample typeSuccess Rate
(50 rfu Threshold)
Success Rate
(150 rfu Threshold)
Number of Samples Tested
Blood on FTA Card99.4-100%
Mean: 99.5%
Mean: 97.3%
Buccal on FTA Card91.8-99.4%
Mean: 97.1%
Mean: 90.9%
Buccal on Bode Buccal
DNA Collector Card
Mean: 97.8%
Mean: 94.0%

Table 1: As part of its rigorous development, the Identifiler Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess “first pass” success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA Cards and Buccal DNA Collectors were amplified with the Identifiler Direct Kit, and the resulting data was analyzed using GeneMapper ID-X, at two different minimum peak amplitude thresholds.

The Confidence to Do More
The Identifiler Direct Kit is part of a new generation of AmpFLSTR Kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler Direct Kit will be able to significantly expand their throughput capabilities, while also maximizing sample integrity and quality assurance for the entire process.

Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR STR Kits, instrumentation, software, and consumables.

The Identifiler Direct Kit is part of a comprehensively validated system for STR-based Human Identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. Validation experiments for the Identifiler Direct Kit were performed by our scientists according to the DNA Advisory Board (DAB) Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods (SWGDAM) Revised Validation Guidelines. However, each laboratory using the Identifiler Direct Kit should perform its own internal validation studies.

The Identifiler Direct Kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems Human Identification solutions in your laboratory.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.


AmpliTaq Gold DNA Polymerase
For Use With (Equipment)
3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer
Label or Dye
Product Line
AmpFLSTR, Identifiler
Product Type
Direct PCR Amplification Kit
Shipping Condition
Dry Ice
D21S11, CSF1PO, vWA, D8S1179, TH01, D18S51, D5S818, D16S539, D3S1358, D2S1338, TPOX, FGA, D7S820, D13S317, Amelogenin, D19S433
For Use With (Application)
STR-based Human Identification
Sample Type
Blood Samples, Buccal Sample, Forensic Sample, Whatman FTA Cards Samples, Blood

Contents & Storage

• AmpFLSTR Identifiler Direct Master Mix
• AmpFLSTR Identifiler Direct Primer Set
• AmpFLSTR Identifiler Direct Control DNA 9947A
Store at -20 °C upon receipt, 2 to 8 °C after initial use

• AmpFLSTR Identifiler Direct Allelic Ladder
Store at -20 °C upon receipt, 2 to 8 °C after initial use


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    Frequently asked questions (FAQs)

    Citations & References

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