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The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating was developed for the AFNOR-approved MicroSEQ® Listeria species and Listeria monocytogenes Real-Time PCR detection workflows. A spin, transfer, and bead beating protocol efficiently lyses Listeria from a 750 µL aliquot of more challenging enriched food samples (e.g., samples containing high fat or otherwise prone to PCR inhibition). The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating meets the needs of lower throughput food laboratories.
• Designed for Food Safety and Environmental Monitoring applications • Supports low-to-medium throughput (up to 100 samples a day) • Inactivates PCR inhibitors and produces high quality DNA for downstream detection • Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from the most challenging sample types.
Designed for Laboratories that Prepare up to 100 Samples a Day A spin column-based preparation enables small batch sizes to be managed while requiring only simple benchtop devices to process, thereby limiting the capital expenditure associated with much higher throughput automation devices.
Removes PCR Inhibitors and Produces High Quality DNA The PrepSEQ® Rapid Spin Sample Prep Kit - Extra Clean & Bead Beating offers a simple, cost-effective way to prepare Listeria DNA from more difficult starting matrices such as cheese, milk, and high fat meats. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube. The pellet is resuspended, then transferred (to avoid carryover, which inhibits PCR) to a new tube containing zirconium beads for mechanical lysis.
For Testing of Food and Environmental Samples Only.
High-throughput Compatible, Automated Protocols
Food & Environmental Samples
Contents & storage
Store lysis buffer in refrigerator (2–8°C). DO NOT FREEZE.