Thermo Scientific™

POROS™ CaptureSelect™ IgM Affinity Column, 4.6 x 50 mm

Catalog number: 4469164
Thermo Scientific™

POROS™ CaptureSelect™ IgM Affinity Column, 4.6 x 50 mm

Catalog number: 4469164
Catalog Number
Unit Size
1 column
Price (USD)
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44691641 column
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Product Overview
Citations & References
Additional Information
Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

POROS™CaptureSelect™ IgM HPLC columns incorporate the advantages of high-speed Poros chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three minute quantitation of IgM antibodies.


• Broad utility—POROS™ A, G and CaptureSelect™affinity ligands address most biological drug classes
• Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—deliver high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ IgM affinity ligands when combined with POROS™ 20 µm resin provides a high flow rate, high performance chromatography solution for the rapid quantitation and small-scale purification of IgM.

POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis including mass spectrometry.

POROS™ affinity chromatography columns are suitable for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS™ Affinity Chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

LC Lambda Column Specifications:
Protein Binding Capacity 4 mg/mL
Species Specifiity Human, Mouse, Rat
Column Material PEEK™ polymer or stainless steel
Frit 2 µm, titanium
POROS™ Resin Cross-linked poly (styrenedivinylbenzene)
CaptureSelect™ Ligands Single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae

For Research Use Only. Not for use in diagnostics procedures.


1 Column
Stationary Phase
CaptureSelect™ IgM
Column Type
Affinity, Prepacked
Product Line
CaptureSelect™, POROS™
IgM Antibodies
Affinity Column
Capacity (Metric)
0.8 mL
Flow Rate
Up to 5000 cm/hr.
Pore Size
0.16 micron
Binding Strength
4.0 mg/ml dynamic binding capacity
Diameter (Metric)
4.6 mm
Elution Volume Max
1.6 ml
Elution Volume Min
800 µl
For Use With (Equipment)
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Max. Operating Pressure
180 bar
Length (Metric)
50 mm
Shipping Condition
Wet Ice
Human, Mouse, Rat
Sample Type
Cell Culture Supernatants
Particle Size
20 micron

Contents & Storage

Column includes EZ grip fittings and column test certificate

Store at 4°C DO NOT FREEZE