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Ion AmpliSeq™ Cancer Panel Primer Pool

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Description

The Ion AmpliSeq™ Cancer Panel Primer Pool is a single pool of primers used to perform multiplex PCR for preparation of amplicon libraries from genomic "hot spot" regions that are frequently mutated in human cancer genes. This primer pool contains the same primers included in the Ion AmpliSeq™ Cancer Panel (download the Ion AmpliSeq™ Cancer Panel Mutations List of targeted genes and mutations), but is now sold separately for use with the improved Ion AmpliSeq™ Library Kit 2.0.

Features of the Ion AmpliSeq™ Cancer Panel Primer Pool include:

• Same coverage as the Ion AmpliSeq™ Cancer Panel
• Improved performance when used with Ion AmpliSeq™ Library Kit 2.0
• More streamlined workflow, higher coverage uniformity, and less bias

Same Relevant Content as Ion AmpliSeq™ Cancer Panel Kit
Like the Ion AmpliSeq™ Cancer Panel, this primer pool is designed to amplify 190 amplicons covering 739 COSMIC mutations in 604 loci from 46 oncogenes and tumor suppressor genes with emphasis on variants in KRAS, BRAF, and EGFR for the detection of somatic mutations in archived cancer samples.

Improved Performance with Ion Ampliseq™ Library Kit 2.0
For improved performance over the Ion AmpliSeq™ Cancer Panel, the Ion AmpliSeq™ Cancer Primer Pool should be used with the improved Ion AmpliSeq™ Library Kit 2.0 for a more streamlined workflow, higher coverage uniformity, and less bias with the option of amplification-free libraries. For completion of projects already in progress and for bridging studies, the Ion AmpliSeq™ Cancer Panel is still available in limited quantities.

Simplicity with Low DNA Input from FFPE Samples
As with the Ion AmpliSeq™ Cancer Panel, the Ion AmpliSeq™ Cancer Primer Pool plus Ion AmpliSeq™ Library Kit 2.0 can be used for targeted library construction with as little as 10 ng of input DNA, enabling cancer genetic studies from archived samples that contain restricted amounts of DNA, such as FFPE tissues.

Single Day Workflow
Ion AmpliSeq™ technology enables the fastest time to targeted libraries, with target selection and library construction completed in approximately 3.5 hours. This revolutionary single-tube workflow is as simple as setting up a PCR reaction.

Scalability with Multiplexing
To leverage improvements in number of reads and accuracy, resulting targeted libraries should be used with the Ion OneTouch™ 200 Template Kit and the Ion PGM™ 200 Sequencing Kit for downstream template preparation and sequencing, respectively. Sequencing is then performed on the Ion PGM™ Sequencer, and soon, the Ion Proton™ Sequencer. Multiple samples can be simultaneously tested on a single Ion 316™ or Ion 318™ Chip with the use of the Ion Xpress™ Barcode Adapter kits. Mutations in KRAS, BRAF, and EGFR are covered at a minimum of 500X depth of coverage (on average >2,000X).

The intuitive Torrent Suite Software and Torrent Variant Caller Plugin enables you to go from extracted DNA to variant calls in as little as 10 hours. Ion Reporter™ Software can then be used for annotation of variants in routine DNA assays for the advancement of clinical research.

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion PGM™ System
Library Type: Targeted Sequencing Library
Workflow Step: Target Selection
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Species: Human
Technique: Post-Light™ Ion Semiconductor Sequencing, Amplicon Sequencing
Product Size: 10 reactions
Multiplex Capability: Multiplex PCR: 190 amplicons
Sample Type (Specific): Double-Stranded DNA (dsDNA), Clinical Sample, Cell Cultures, Blood, DNA (Genomic), Fine Needle Aspirates (FNA), Whole Blood, Tissue (FFPE)
Starting Material (Amount): 10 ng DNA

Contents & storage

1 tube. Store in refrigerator (2–8°C).

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Manuals & protocols

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