Customer Notice: Please be sure to follow the primer concentration guidelines as specified in the User Guide. For example, if you are using SYBR® Select with a StepOne™ or StepOnePlus™ Real-Time PCR Instrument under Standard Mode, please use a primer concentration less than 200 nM. Also, for these two instruments, please note that new StepOne™ Software v2.3 is also available for download.
Applied Biosystems™

SYBR™ Select Master Mix

Catalog number:  4472918

 Related applications:

Real Time PCR (qPCR)

| Real Time PCR-Based Gene Expression Profiling

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SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

Features of the SYBR® Select Master Mix include:

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR Select Master Mix is specially formulated to provide robust results at 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR® Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.

View performance data.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.


Detection Method: SYBR
For Use With (Equipment): 7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Reaction Speed: Fast, Standard
PCR Method: qPCR
Sample Type (General): DNA
Form: Liquid
Format: Vial(s)
Hot Start: Built-In Hot Start
Label or Dye: SYBR® GreenER™
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq® DNA Polymerase
Primer-Probe Compatibility: Unlabeled Oligos
Product Line: SYBR®
Product Size: 2 x 5 mL
Purity or Quality Grade: UP (Ultra Pure)
Sample Type (Specific): DNA (Genomic), cDNA

Contents & storage

The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 µL reactions (10 µL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.


Manuals & protocols