SYBR® Select Master Mix

Description

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis.

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SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

Features of the SYBR® Select Master Mix include:

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR® Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.

View performance data.

Figures

Specifications

Detection Method:	SYBR
For Use With (Equipment):	7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Reaction Speed:	Fast, Standard
PCR Method:	qPCR
Sample Type (General):	DNA
Form:	Liquid
Format:	Vial(s)
Hot Start:	Built-In Hot Start
Label or Dye:	SYBR® GreenER™
Passive Reference Dye:	ROX (Pre-mixed)
Polymerase:	AmpliTaq® DNA Polymerase
Primer-Probe Compatibility:	Unlabeled Oligos
Product Line:	SYBR®
Product Size:	2 x 5 mL
Purity or Quality Grade:	UP (Ultra Pure)
Sample Type (Specific):	DNA (Genomic), cDNA

Contents & storage