TaqMan® miRNA ABC Purification Kit - Human Panel A This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 4473087

Applied Biosystems™  Related applications: RNA Extraction | miRNA & Non-Coding RNA Analysis

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Description

Use the TaqMan® miRNA ABC Purification Kit to purify high-quality miRNA from blood-related or other difficult sample types without hazardous chemicals.
• Simple and fast workflow using oligonucleotide-conjugated Dynal® magnetic beads
• Small sample input: typical volumes range from 10 µL for blood samples to 50 µL for serum and plasma samples
• RT-PCR inhibitors commonly found in blood-related samples are easily washed away
• No need for hazardous chemicals such as phenol, chloroform, Trizol® reagent, or guanidine isothiocyanate

Simple and Fast miRNA Purification
The TaqMan® miRNA ABC (anti-miRNA bead capture) purification kit contains buffers and magnetic beads for single-tube isolation of specific microRNA (miRNA) from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes (see workflow, Figure 1).

Human Panel A and Human Panel B
The TaqMan® miRNA ABC Purification Kit is offered in two configurations: Human Panel A and Human Panel B. The beads in each kit are superparamagnetic Dynabeads® covalently bound to a unique set of 377 anti-miRNA oligonucleotides for each panel that correspond to the assays contained in the TaqMan® Array Human MicroRNA A and B cards. The miRNA isolation process relies on hybridization of miRNAs to the corresponding anti-miRNA oligonucleotides attached to the beads. Complete lists of miRNAs isolated by the panels can be found in the TaqMan® miRNA ABC Purification Kit protocol (below).

miRNA Analysis
The miRNAs isolated using the TaqMan® miRNA ABC Purification Kit are ready for conversion into cDNA and downstream analysis by quantitative PCR using either single tube (individual) TaqMan® microRNA assays or the TaqMan® Array Human microRNA A and B cards. Figure 2 gives examples of the data that can be obtained from miRNA isolated from various sample types using the TaqMan® miRNA ABC Purification Kit.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Liquid Samples (e.g. Serum), Tissue, FFPE & Fixed Samples, Cells, Blood
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Real-Time Quantitative PCR (qPCR), microRNA Analysis
System: Dynabeads®
Species: Human
Product Size: 40 preps
Final Product: micro RNA
Purification Time: 75 min
Isolation Technology: Magnetic Bead
Sample Type (Specific): Plasma, Tissue (FFPE), Any Tissue Type, Whole Blood, Saliva, Urine, Serum, Cell Cultures
Green Features: Less hazardous, Less waste, Fewer resources used

Contents & storage

Buffer Kit - Store at room temperature
Lysis Buffer
ABC Buffer
Wash Buffer 1
Wash Buffer 2
Elution Buffer
1.5 mL LoBind tubes

Human Panel A Beads
Store at 2 °C to 8 °C

Documents

Manuals & protocols

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