Applied Biosystems™

TaqMan™ miRNA ABC Purification Kit - Human Panel A

Green features
Catalog number: 4473087
Applied Biosystems™

TaqMan™ miRNA ABC Purification Kit - Human Panel A

Green features
Catalog number: 4473087
Catalog Number
4473087
Unit Size
40 preps
Price (USD)
445.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
447308740 preps
445.00
Your Price:
***
Product Overview
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FAQ
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Additional Information
Recommendations
Recommendations
Use the TaqMan™ miRNA ABC Purification Kit to purify high-quality miRNA from blood-related or other difficult sample types without hazardous chemicals.
• Simple and fast workflow using oligonucleotide-conjugated Dynal™ magnetic beads
• Small sample input: typical volumes range from 10 µL for blood samples to 50 µL for serum and plasma samples
• RT-PCR inhibitors commonly found in blood-related samples are easily washed away
• No need for hazardous chemicals such as phenol, chloroform, Trizol™ reagent, or guanidine isothiocyanate

Simple and Fast miRNA Purification
The TaqMan™ miRNA ABC (anti-miRNA bead capture) purification kit contains buffers and magnetic beads for single-tube isolation of specific microRNA (miRNA) from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes (see workflow, Figure 1).

Human Panel A and Human Panel B
The TaqMan™ miRNA ABC Purification Kit is offered in two configurations: Human Panel A and Human Panel B. The beads in each kit are superparamagnetic Dynabeads™ covalently bound to a unique set of 377 anti-miRNA oligonucleotides for each panel that correspond to the assays contained in the TaqMan™ Array Human MicroRNA A and B cards. The miRNA isolation process relies on hybridization of miRNAs to the corresponding anti-miRNA oligonucleotides attached to the beads. Complete lists of miRNAs isolated by the panels can be found in the TaqMan™ miRNA ABC Purification Kit protocol (below).

miRNA Analysis
The miRNAs isolated using the TaqMan™ miRNA ABC Purification Kit are ready for conversion into cDNA and downstream analysis by quantitative PCR using either single tube (individual) TaqMan™ microRNA assays or the TaqMan™ Array Human microRNA A and B cards. Figure 2 gives examples of the data that can be obtained from miRNA isolated from various sample types using the TaqMan™ miRNA ABC Purification Kit.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Specifications

Format
Kit
Isolation Technology
Magnetic Bead
Purification Time
75 min.
RNAi Type
miRNA
Species
Human
Sample Type
Cell Cultures, Plasma, Saliva, Serum, Tissue, Tissue (FFPE), Tissue Sections, Urine, Whole Blood
Final Product Type
micro RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
Real-Time Quantitative PCR (qPCR), microRNA Analysis
Green Features
Fewer resources used and less waste, Less hazardous
Product Line
TaqMan™
Product Type
miRNA ABC Purification Kit
Quantity
40 Preps
Target
miRNA
System Type
Dynabeads™

Contents & Storage

Buffer Kit - Store at room temperature
Lysis Buffer
ABC Buffer
Wash Buffer 1
Wash Buffer 2
Elution Buffer
1.5 mL LoBind tubes

Human Panel A Beads
Store at 2 °C to 8 °C

Figures