PrepFiler® Forensic DNA Extraction Kit, updated, with plastics

Catalog number: 4473850

Applied Biosystems™  Related applications: Forensics

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The PrepFiler® Forensic DNA Extraction Kit uses magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types, including body fluids, and stains and swabs of body fluids. This kit is designed to help improve the overall yield and concentration of DNA recovered from both routine and challenging samples, while enabling removal of PCR inhibitors. This means greater quantities of highly purified DNA for improved downstream genotyping results. This kit is intended for manual use with 1.5 mL tubes. For automated workflows, please see the PrepFiler® Automated Forensic DNA Extraction Kit.

• Designed to improve the overall yield, concentration, and purity of DNA isolated from both routine and challenging forensic samples
• Contains specially developed magnetic particles and reagents that optimize DNA binding and elution of DNA in a small volume
• Offers streamlined protocols to process an extensive variety of forensic samples
• Part of an integrated solution including novel reagents, consumables, and automated options to enable reliable, simplified, and time-saving DNA purification
• Improves downstream genotyping (STR analysis) success rate, to help ensure that critical and often limited casework samples can be successfully analyzed

Improved Packaging and Configuration
Several packaging and configuration improvements have been implemented for the updated PrepFiler® kits:
• An additional wash buffer, PrepFiler® Wash Buffer B, has now been incorporated into the kits to minimize the potential for detergent carryover from PrepFiler® Wash Buffer A, which can inhibit downstream PCR applications when the maximum volume of DNA extract is used for subsequent PCR amplification.
• The kit packaging has been updated to include all reagents, including magnetic particles, in the same kit box, with a single expiration date.
• Shipping will occur at ambient temperature, and the kits can also be stored upon receipt at ambient temperature.
• In this kit configuration, plastic consumables are included (PrepFiler® Filter Columns for substrate removal and PrepFiler® Spin Tubes for sample processing in tube format). For the kit without plastics, see Cat. No. 4463351.

Package components

Unit size


PrepFiler® Spin Tubes and Filter Columns (300 tubes/100 columns) Catalog number:4392342 100 columns 1
PrepFiler® Forensic DNA Extraction Kit, updated Catalog number:4463351 1 kit 1
For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.


Sample Type (General): Forensic Samples
Downstream Application: Forensic Testing
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Size: 100 reactions
Shipping Condition: Room Temperature


Manuals & protocols