Ion OneTouch™ 2 Instrument

Catalog number: 4474778

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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Description

The Ion OneTouch 2 Instrument performs template amplification as part of the manual workflow for the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. The instrument has a small footprint and fits easily on any bench top.

For a fully automated workflow, please see the Ion Chef Instrument.

The Ion OneTouch ES enrichment module is not included in this purchase. For both the Ion OneTouch 2 Instrument and ES module, please see the Ion OneTouch 2 System.

Efficiency and flexibility
The Ion OneTouch 2 instrument integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion PGM, up to 200 bases on the Ion Proton System, up to 400 bases on the Ion 520 and Ion 530 chips, and up to 200 bases on the Ion 540 chip. The need for gas is eliminated by the use of a peristaltic pump.

Contributing technologies
At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the OneTouch2 instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint.

Designed to fit in any lab
Coupled with the Ion OneTouch ES enrichment module (not included) the Ion OneTouch 2 Instrument enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment.

Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chip Compatibility: Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, Ion 318™ Chip v2 BC, Ion 520™ Chip, Ion 530™ Chip, Ion 540™ Chip,  Ion PI™ Chip v3
Dimensions: 12 x 16 x 14 in (30 x 41 x 36 cm)
For Use With (Equipment): Ion PGM™ System, Ion Proton™ System, Ion S5™ System, Ion S5™ XL System
High Throughput Compatibility: High Throughput-Compatible, Manual Protocols, Multiplexing
Library Type: Fragment Library, Mate-Paired Library, Targeted Sequencing Library, cDNA Library
Multiplex Capability: Up to 384 barcoded libraries
Operating Environment: Humidity: 20%-80% (non-condensing), Temperature: 15°C-25°C
Product Line: Applied Biosystems™, Ion OneTouch™
Product Size: 1 system
Run Time: Hands-on time: 1 - 1.5 hours, Instrument run time: 4.8 - 6.5 hours
Shipping Condition: Room Temperature
Voltage Limit: International: 220 V, US: 110 V
Weight: 23 lb (10.4 kg)

Documents

Manuals & protocols