Ion OneTouch™ 2 Instrument

Catalog number: 4474778

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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The Ion OneTouch™ 2 Instrument performs template amplification as part of the manual workflow for the Ion PGM™, Ion Proton™, and Ion S5™ and S5™ XL systems. For a fully automated workflow, please consider the Ion Chef™ Instrument. The Ion OneTouch™ 2 ES enrichment module is not included in this purchase. For both the Ion OneTouch 2 Instrument and ES module, please see the Ion OneTouch™ 2 System.

The Ion OneTouch 2 Instrument:
• Integrates multiple manual template preparation steps into a single system
• Enables parallel processing of multiple samples per day through modular design
• Supports scalable template preparation for all Ion semiconductor sequencing chips
• Supports up to 400 bases on the Ion PGM System, up to 200 bases on the Ion Proton System, and up to 400 bases on the Ion 520™/530™ chips and up to 200 bases on the Ion 540™ Chip
• Employs a peristaltic pump fluidic system that eliminates the need for gas
• Fits easily on any bench top with its small instrument footprint

At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of templated Ion Sphere™ particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate system that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the templated Ion Sphere particles. Combined, these first-in-class technologies deliver massively parallel clonal amplification and recovery with walk-away operation—all in a small bench top footprint.

The modular design of the Ion OneTouch 2 Instrument/System enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, and Ion S5 and S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of the Ion semiconductor sequencing and the Ion OneTouch 2 Instrument/System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of Ion Sphere particles, to maximize experimental control and the success of each experiment.

For Research Use Only. Not for use in diagnostic procedures.


Chip Compatibility: Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, Ion 318™ Chip v2 BC, Ion 520™ Chip, Ion 530™ Chip, Ion 540™ Chip,  Ion PI™ Chip v3
Dimensions: 12 x 16 x 14 in (30 x 41 x 36 cm)
For Use With (Equipment): Ion PGM™ System, Ion Proton™ System, Ion S5™ System, Ion S5™ XL System
High Throughput Compatibility: High Throughput-Compatible, Manual Protocols, Multiplexing
Library Type: Fragment Library, Mate-Paired Library, Targeted Sequencing Library, cDNA Library
Multiplex Capability: Up to 384 barcoded libraries
Operating Environment: Humidity: 20%-80% (non-condensing), Temperature: 15°C-25°C
Product Size: 1 system
Run Time: Hands-on time: 1 - 1.5 hours, Instrument run time: 4.8 - 6.5 hours
Shipping Condition: Room Temperature
Voltage Limit: International: 220 V, US: 110 V
Weight: 23 lb (10.4 kg)


Manuals & protocols