Ion OneTouch™ 2 System

Description

The Ion OneTouch 2 System performs template amplification and enrichment as part of the manual workflow for the Ion PGM, Ion Proton, and Ion S5 and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. With its small footprint, the system fits easily on any bench top.

For a fully automated workflow, please see the Ion Chef Instrument.

Efficiency and flexibility
The Ion OneTouch 2 System integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM System, and up to 200 bases on the Ion Proton System. The need for gas is eliminated by the use of a peristaltic pump.

System components
The Ion OneTouch 2 System consists of two modules: the Ion OneTouch 2 Instrument and the Ion OneTouch ES (Enrichment System). The Ion OneTouch 2 Instrument may be purchased separately.

Contributing technologies
At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint.

Designed to fit in any lab
With the included Ion OneTouch ES module, the Ion OneTouch 2 System enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment.

Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

Specifications

Chip Compatibility:	Ion 314™ Chip, Ion 316™ Chip, Ion 318™ Chip, Ion PI™ Chip
Dimensions:	Ion OneTouch™ 2 Instrument: 12 x 16 x 14 in (30 x 41 x 36 cm), Ion OneTouch™ ES: 9.5 x 12.5 x 11 in (24 x 32 x 28 cm)
For Use With (Equipment):	Ion OneTouch™ 2 System, Ion PGM™ System, Ion Proton™ System
High Throughput Compatibility:	Automated Protocols, High Throughput-Compatible, Multiplexing
Library Type:	Fragment Library, Mate-Paired Library, Targeted Sequencing Library, cDNA Library
Multiplex Capability:	Up to 384 barcoded libraries
Operating Environment:	Humidity: 20%-80% (non-condensing), Temperature: 15°C-25°C
Product Line:	Ion OneTouch™
Product Size:	1 system
Run Time:	Hands-on time: Minutes, Ion PGM™ System: 5 hours total for template preparation, Ion Proton™ System: 8 hours total for template preparation
Voltage Limit:	International: 220 V, US: 110 V
Weight:	Ion OneTouch™ 2 Instrument: 23 lb (10.4 kg), Ion OneTouch™ ES: 12 lbs (5.4 kg)

Contents & storage