Invitrogen™

DynaLight™ Substrate with RapidGlow™ Enhancer

Catalog number:  4475406

Related applications:

Protein Assays and Analysis

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Description

Achieve faster ELISA results with DynaLight™ Substrate with RapidGlow™ Enhancer, a ready-to-use chemiluminescent substrate formulation that has been optimized to achieve faster results in solution-based assays. The DynaLight™ Substrate with RapidGlow™ Enhancer formulation includes 1,2-dioxetane chemiluminescent substrate and a polymeric enhancer that enables ultra-sensitive immunoassay detection by alkaline phosphatase label.

Features of DynaLight™ Substrate with RapidGlow™ Enhancer:

• Faster kinetics and higher signal levels than colorimetric, fluorescent, or other chemiluminescent chemistries
• Sensitive attomole detection of purified alkaline phosphatase enzyme
• Optimized formulation for both microplate and bead-based assays
• Substrate stability of over 1 year at 4°C
• Compatibility with automated platforms with trigger solution/positions already in place

The DynaLight™ Substrate with RapidGlow™ Enhancer provides a robust chemiluminescent reaction when catalyzed by alkaline phosphatase. This reaction achieves maximum light emission within 2 minutes at 37°C and maintains a sustained signal for hours. When combined with DynaLight™ Trigger Solution, DynaLight™ Substrate with RapidGlow™ Enhancer delivers maximum signal within seconds. The environmentally-friendly DynaLight™ Trigger Solution provides maximum signal within 1 second of addition, with a longer half-life to its ground state than other chemiluminescent Flash technologies, allowing ample time to collect data reliably.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Specifications

Assay: Immunoassay
Compatible Cells: Mammalian Cells
Detection Limit: pg/ml to µg/ml
Form: Liquid
High-throughput Compatibility: High-throughput Compatible
Product Line: DynaLight™, NovaBright™, RapidGlow™
Reaction Time: 2 min
Readout: End Point
Shipping Condition: Wet Ice
Substrate Properties: Chemical Substrate
Target Enzyme: Alkaline Phosphatase
Detection Method: Chemiluminescent
Substrate Type: AP (Alkaline Phosphatase) Substrate
For Use With (Equipment): Luminometer (Microplate)
Format: Bottle(s)
Quantity: 1 bottle(s)
Sample Type: Cell Cultures, Whole Blood, Cultured Cells, Serum
Sensitivity: 1 pg⁄ml
Substrate: DynaLight™ Substrate, Alkaline Phosphatase

Contents & storage

1 amber bottle — Store at 2 to 8°C