Ion Plus Fragment Library Kit for AB Library Builder™ System

Description

The Ion Plus Fragment Library Kit for AB Library Builder™ System provides a rapid, flexible, and high-throughput solution for library construction upstream of semiconductor sequencing when starting with fragmented DNA. This ready-to-use reagent cartridge simplifies next-generation sequencing by enabling automated DNA fragment library preparation for the Personal Genome Machine® and soon the Proton™ sequencing systems.

This kit contains sample preparation reagents for library construction of 13 DNA libraries for semiconductor sequencing. Use of this reagent cartridge requires additional standard or barcoded library adapters (sold separately).

This library kit offers:

• Simplicity and convenience of automated library preparation upstream of semiconductor sequencing
• Rapid and flexible workflow automating library creation and size selection in as little as 2 hours starting with pre-fragmented DNA
• Lower input requirement providing the ability to make amplification-free libraries from as little as 50 ng starting material
• Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

With greatly reduced hands-on time, the Ion Plus Fragment Library Kit for AB Library Builder™ System automates preparation of up to 13 standard, paired-end, or barcode fragment libraries in about 2 hours per run, starting with small amplicons or pre-sheared gDNA samples. Size selection of the libraries is performed automatically without the expense or effort of gel-based methods. Alternatively, libraries can be manually size-selected by conventional methods after the automated run.

Starting with 50–1000 ng of input DNA, the kit yields significantly higher amounts of library than current manual methods, eliminating the need for PCR amplification (i.e. 'amplification-free' libraries) for most sequencing needs.

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With the ability to prepare up to 13 libraries in a little over 2 hours, the Ion Plus Fragment Library Kit for AB Library Builder™ System is a key step towards automation of the Ion sequencing workflow, making comprehensive analyses of your sample(s) of interest more convenient than ever before. This modular reagent system allows for the construction of a variety of fragment libraries (i.e., standard and barcoded) depending on the library adapters used (library adapters sold separately).

Ion Torrent Sequencing Made Simpler, Faster, and More Affordable
The Ion Plus Fragment Library Kit for AB Library Builder™ System automates library creation and size selection, eliminating the need for costly time-consuming gel-based size selection, and requires as little as 50 ng of sample, accelerating your path to semiconductor sequencing. This automated library preparation solution provides fast and scalable workflows for sequencing projects requiring low- to high-throughput processing capabilities.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Specifications

For Use With (Equipment):	Ion PGM™ System, AB Library Builder™
Sample Type:	DNA (Pre-Sheared)
High-throughput Compatibility:	High-throughput Compatible, Automated Protocols
Libraries:	Fragment Library
Sequencing Type:	Genome & DNA Sequencing
Starting Material:	DNA
Starting Material Amount:	As little as 50 ng DNA
Workflow Step:	Library Generation
Product Line:	Ion Torrent™

Contents & storage