Ion TargetSeq™ Exome Kit - FAQs

The Ion TargetSeq Exome Enrichment Kit utilizes >4 probes per exon, as opposed to 1-2 probes for alternative technologies.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion TargetSeq Exome workflow offers standard and accelerated protocols to generate a library in 4 or 2 days, respectively. Suggested timelines for generating TargetSeq Exome Libraries are shown below;

Standard protocol timeline;
Day 1: Prepare library, amplify and purify, quanitfy, set up and start hybridization (10 hrs)
Days 2-4: Hybridization at 47 degrees C (64-72 hrs)
Day 4: Wash and recover beads with enriched DNA, amplify and purify, quantify the enriched library (5 hrs)

Accelerated protocol timeline;
Day 1: Prepare library, amplify and purify, quanitfy, setup and start hybridization (10 hrs)
Night 1: Hybridization at 47 degrees C (16-18 hrs)
Day 2: Wash and recover beads with enriched DNA, amplify and purify, quantify the enriched library (5 hrs)

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

We recommend starting with 1 µg of high quality, RNA-free genomic DNA for the best results. Libraries have been generated with inputs as low as 100 ng, but at the cost of reduced complexity. Please speak with your local Field Applications Scientist for further details.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion TargetSeq Exome Enrichment Kit targets ~49.6 Mb with the following features:
> 21,500 protein coding genes
>8,000 RNA genes (miRNA, snoRNA, tRNA, and other ncRNA)
>44,000 predicted miRNA binding sites
>33,500 COSMIC positions

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.