Total Exosome Isolation Reagent (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples. • Maximize recovery of intact exosomes for any type of downstream application • Easily isolate exosomes using our simple and reliable protocol • Avoid time-consuming ultra-centrifugation • Maximize flexibility—can be scaled up or down depending on the sample size
Isolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation Reagent (from cell culture media) enables simple and reliable concentration of intact exosomes from cell media samples with a protocol that is scalable depending on your sample size.
By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the cell media sample, and the solution is incubated overnight at 2°C to 8°C. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 60 min. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can then be purified using the Total Exosome RNA and Protein Isolation Kit, or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking. For immunoprecipitation of exosomal proteins, use Exosome Immunoprecipitation (Protein A) or Exosome Immunoprecipitation (Protein G).