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Ion Torrent™

Ion AmpliSeq™ Sample ID Panel

Catalog number:  4479790

Related applications:

Oncology & Genetic Disease Research

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The Ion AmpliSeq™ Sample ID Panel is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 specially designed primer pairs that can be added to the multiplex PCR reaction to generate a unique ID during post-sequencing analysis of research samples.

Designed to advance clinical research, the Ion AmpliSeq™ Sample ID Panel helps reveal sample misidentification for increased confidence during data analysis and reporting. The Ion AmpliSeq™ Sample ID Panel can be used in conjunction with any Ion AmpliSeq™ ready-to-use panel or custom panel designed via the Ion AmpliSeq™ Designer (

Verify Various Sample Combinations:

• Tumor/normal paired samples
• Samples from the same individual in longitudinal studies
• Multi-tissue or multi-tumor samples from the same individual

Sample Identification Through SNP Genetyping

• 8 primer pairs target 8 validated SNPs1
• Selected SNPs are unlinked, non-exonic markers that exhibit exceptional robustness and represent high minor-allele frequency in all human populations
• An additional primer pair targets the amelogenin gene, allowing quick gender determination
• The first letter of the Sample ID code provides instant gender determination for the sample
• Optimal discrimination power (~1:5,000*) with minimal sequencing needed

Simple Workflow—Just One Pipetting Step

• Spike 1 µL of the 20X Sample ID primer pool directly into any Ion AmpliSeq™ primer pool for co-amplification and downstream sequencing and data analysis
• Enabled for use with any Ion Xpress™ barcode
• Compatible with the Ion Reporter™ Software, a cloud-hosted software tool for automated variant analysis

20X Ion AmpliSeq™ Sample ID Primer Pool

Torrent Suite Software (v3.0 or greater) that includes the Ion AmpliSeq™ Sample ID Panel plug-in
Ion AmpliSeq™ Library Kit 2.0 User Guide
Ion AmpliSeq™ ready-to-use panel or custom panel designed via the Ion AmpliSeq™ Designer (

1Pakstis AJ et al. SNPs for a Universal Individual Identification Panel. Hum Genet. 2010 Mar; 127(3):315-24.
*4641 discrimination power calculations assumes no missing genotyping data

For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System, Ion Proton™ System
Species: Human
Primer: Sample ID Primers
Libraries: Targeted Sequencing Library
Multiplex Capability: 9 amplicons
Sequencing Type: Genome & DNA Sequencing
Shipping Condition: Approved for shipment at Room Temperature or on Dry Ice
Starting Material: DNA
Starting Material Amount: 10 ng gDNA
Technique: Amplicon Sequencing
Workflow Step: Sample QC
Product Line: Ion AmpliSeq™

Contents & storage

1 tube 20X Ion AmpliSeq™ Sample ID Primer Pool
Store at -30 °C to -10 °C