Need help deciding what product to use with the Ion PGM™ System? Check out our helpful Applications Decision Tree for the Ion PGM™ System.

Ion AmpliSeq™ Sample ID Panel

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Ion AmpliSeq™ Sample ID Panel is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 specially designed primer pairs that can be added to the multiplex PCR reaction to generate a unique ID during post-sequencing analysis of research samples.

Designed to advance clinical research, the Ion AmpliSeq™ Sample ID Panel helps reveal sample misidentification for increased confidence during data analysis and reporting. The Ion AmpliSeq™ Sample ID Panel can be used in conjunction with any Ion AmpliSeq™ ready-to-use panel or custom panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com).

Verify Various Sample Combinations:

• Tumor/normal paired samples
• Samples from the same individual in longitudinal studies
• Multi-tissue or multi-tumor samples from the same individual

Sample Identification Through SNP Genetyping

• 8 primer pairs target 8 validated SNPs1
• Selected SNPs are unlinked, non-exonic markers that exhibit exceptional robustness and represent high minor-allele frequency in all human populations
• An additional primer pair targets the amelogenin gene, allowing quick gender determination
• The first letter of the Sample ID code provides instant gender determination for the sample
• Optimal discrimination power (~1:5,000*) with minimal sequencing needed

Simple Workflow—Just One Pipetting Step

• Spike 1 µL of the 20X Sample ID primer pool directly into any Ion AmpliSeq™ primer pool for co-amplification and downstream sequencing and data analysis
• Enabled for use with any Ion Xpress™ barcode
• Compatible with the Ion Reporter™ Software, a cloud-hosted software tool for automated variant analysis

Includes:
20X Ion AmpliSeq™ Sample ID Primer Pool

Required:
Torrent Suite Software (v3.0 or greater) that includes the Ion AmpliSeq™ Sample ID Panel plug-in
Ion AmpliSeq™ Library Kit 2.0 User Guide
Ion AmpliSeq™ ready-to-use panel or custom panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)

1Pakstis AJ et al. SNPs for a Universal Individual Identification Panel. Hum Genet. 2010 Mar; 127(3):315-24.
*4641 discrimination power calculations assumes no missing genotyping data

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion PGM™ System, Ion Proton™ System
Library Type: Targeted Sequencing Library
Workflow Step: Sample QC
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Species: Human
Technique: Amplicon Sequencing
Product Size: 96 reactions
Multiplex Capability: 9 amplicons
Starting Material (Amount): 10 ng gDNA
Shipping Condition: Approved for shipment at Room Temperature or on Dry Ice

Contents & storage

1 tube 20X Ion AmpliSeq™ Sample ID Primer Pool
Store at -30 °C to -10 °C

Documents

Manuals & protocols