Down-regulated miR-495 can target programmed cell death 10 in ankylosing spondylitis.
AuthorsNi WJ,Leng XM
JournalMolecular medicine (Cambridge, Mass.)
PubMed ID32450789
BACKGROUND: MicroRNAs (miRNAs) play crucial roles in regulating eukaryotic gene expression. Recent studies indicated that aberrantly expressed miRNAs are involved in the pathogenesis of ankylosing spondylitis (AS). Indeed, hsa-miR-495-3p (miR-495) has been reported as an anti-oncogene in different cancers. However, the role of miR-495 in AS is still unknown. METHODS: ... More
Targeting hepatic miR-221/222 for therapeutic intervention of nonalcoholic steatohepatitis in mice.
BACKGROUND: Effective targeting therapies for common chronic liver disease nonalcoholic steatohepatitis (NASH) are in urgent need. MicroRNA-targeted therapeutics would be potentially an effective treatment strategy of hepatic diseases. Here we investigated the functional role of miR-221/222 and the therapeutic effects of antimiRs-221/222 in NASH mouse models. METHODS: We generated the ... More
Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.
BACKGROUND: Increasing evidence has revealed that circular RNAs (circRNAs) play crucial roles in cancer biology. However, the role and underlying regulatory mechanisms of circFNDC3B in bladder cancer (BC) remain unknown. METHODS: A cell invasion model was established by repeated transwell assays, and invasion-related circRNAs in BC were identified through an ... More
Beta-d-Glucan for Diagnosing Pneumocystis Pneumonia: a Direct Comparison between the Wako β-Glucan Assay and the Fungitell Assay.
LRRK2 Contributes to Secondary Brain Injury Through a p38/Drosha Signaling Pathway After Traumatic Brain Injury in Rats.
AuthorsRui Q,Ni H,Gao F,Dang B,Li D,Gao R,Chen G
JournalFrontiers in cellular neuroscience
PubMed ID29545743
Leucine-rich repeat kinase 2 (LRRK2) is widely expressed in the brain and exerts neurotoxicity in Parkinsonâs disease. The p38/Drosha signaling activation has been reported to increase cell death under stress. This study was designed to investigate the potential role and mechanism of LRRK2 in secondary brain injury after traumatic brain ... More
Dendritic cell recognition by group 3 innate lymphoid cells through DNAX accessory molecule 1 triggers proinflammatory reciprocal cell activation.
AuthorsCampana S,Di Carlo E,De Pasquale C,Barberi C,Oliveri D,Migliore GS,Cannavò SP,Galletti B,Pende D,Carrega P,Ferlazzo G
JournalThe Journal of allergy and clinical immunology
PubMed ID31247267
Annexin A7 Levels Increase in Rats With Traumatic Brain Injury and Promote Secondary Brain Injury.
AuthorsGao F,Li D,Rui Q,Ni H,Liu H,Jiang F,Tao L,Gao R,Dang B
JournalFrontiers in neuroscience
PubMed ID29896083
The incidence of traumatic brain injury (TBI) has been increasing annually. Annexin A7 is a calcium-dependent phospholipid binding protein. It can promote melting of the cell membrane. Recent studies have shown that it plays an important role in atherosclerosis, other cardiovascular diseases, and a variety of tumors. However, few studies ... More
Circular RNA ACVR2A suppresses bladder cancer cells proliferation and metastasis through miR-626/EYA4 axis.
BACKGROUND: Circular RNAs (circRNAs) have been considered to mediate occurrence and development of human cancers, generally acting as microRNA (miRNA) sponges to regulate downstream genes expression. However, the aberrant expression profile and dysfunction of circRNAs in human bladder cancer remain to be investigated. The present study aims to elucidate the potential role ... More
Cyclopropane fatty acid biosynthesis in plants: phylogenetic and biochemical analysis of Litchi Kennedy pathway and acyl editing cycle genes.
AuthorsShockey J,Kuhn D,Chen T,Cao H,Freeman B,Mason C
JournalPlant cell reports
PubMed ID30083958
circRASGRF2 functions as an oncogenic gene in hepatocellular carcinoma by acting as a miR-1224 sponge.
AuthorsWu D,Xia A,Fan T,Li G
JournalMolecular therapy. Nucleic acids
PubMed ID33312757
Circular RNAs (circRNAs) are a class of non-coding RNAs broadly expressed in cells of various species. However, the contributions and molecular mechanisms of circRNAs to hepatocellular carcinoma (HCC) remain largely unknown. In the present study, we compared the expression of circRNAs between five paired HCC and adjacent noncancerous liver (ANL) ... More
CircPTPRA acts as a tumor suppressor in bladder cancer by sponging miR-636 and upregulating KLF9.
AuthorsHe Q,Huang L,Yan D,Bi J,Yang M,Huang J,Lin T
JournalAging
PubMed ID31821171
Growing evidence suggests that circular RNAs (circRNAs) play pivotal roles in cancer progression. In this study, bioinformatic analysis identified a dysregulated circRNA termed circPTPRA in bladder cancer (BC). By using qRT-PCR analysis, we verified that circPTPRA is down-regulated in clinical BC specimens compared with the matched non-tumor samples, while correlation ... More
Detection of EBV DNA in Non-Hodgkin Lymphoma Patients in Bulgaria.
AuthorsKostadinova T,Ivanova LI,Todorova TT,Stoykova Z,Staykov D,Efraim M,Gercheva L
JournalIndian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion
PubMed ID31388258
EpsteinâBarr virus is the first human oncogenic virus associated with a broad range of different malignant diseases but its role in non-Hodgkin lymphomas (NHL) development still needs to be fully understood. High expression levels of EBV major genes are found in NHL tumor cells and free viral DNA circulates in the ... More
Circular RNA circERBB2 promotes gallbladder cancer progression by regulating PA2G4-dependent rDNA transcription.
BACKGROUND: CircRNAs are found to affect initiation and progression of several cancer types. However, whether circRNAs are implicated in gallbladder cancer (GBC) progression remains obscure. METHODS: We perform RNA sequencing in 10 pairs of GBC and para-cancer tissues. CCK8 and clone formation assays are used to evaluate proliferation ability of ... More
Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation.
AuthorsFrans G,Beuselinck K,Peeters B,Van Ranst M,Saegeman V,Desmet S,Lagrou K
JournalDiagnostic microbiology and infectious disease
PubMed ID30929995
The goal of the study was to adapt our Middle East respiratory syndrome coronavirus (MERS-CoV) lab-developed test (LDT) to 3 âSample to Resultâ (S2R) systems: BD MAX (BD), ELITe InGenius (ELITechGroup), and ARIES (Luminex). The BD MAX and InGenius system allowed use of lab-developed primers and TaqMan probes, while ARIES ... More
Circular RNA circUBXN7 represses cell growth and invasion by sponging miR-1247-3p to enhance B4GALT3 expression in bladder cancer.
Circular RNAs (circRNAs) have recently been confirmed to participate in different pathological processes, including cancer progression. However, the role and precise mechanism of action of the majority of circRNAs have not been elucidated in bladder cancer (BC). Here, we identified a novel circular RNA, termed circUBXN7, which was significantly downregulated ... More
Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance.
BACKGROUND: During the fifth wave of human H7N9 infections, a novel highly pathogenic (HP) H7N9 variant emerged with an insertion of multiple basic amino acids in the HA cleavage site. Moreover, a neuraminidase inhibitor (NAI) resistance (R292K in NA) mutation was found in H7N9 isolates from humans, poultry and the ... More
MicroRNA‑212 facilitates the motility and invasiveness of esophageal squamous carcinoma cells.
AuthorsChen Z,Liu Y,Qi B,Gu C,Wei X,Guo L,Yao W,Zhao B
JournalMolecular medicine reports
PubMed ID31485658
As a tumor-associated microRNA (miR), miR-212 has dual functions; either as an oncogene or a tumor suppressor. A high expression level of miR-212 was reported to be associated with poor outcome in patients with esophageal squamous cell carcinoma (ESCC), however, its role in ESCC progression has not been explored. In ... More
Concordance of the 21-gene assay between core needle biopsy and resection specimens in early breast cancer patients.
BACKGROUND: Adjuvant therapy decisions may be partly based on the results of a multigene quantitative reverse transcription-polymerase chain reaction (RT-PCR)-based assay: the 21-gene recurrence score (RS) test of resection specimens. When necessary, core needle biopsy (CNB) may be considered as a surrogate. Here, we evaluated the concordance in gene expression ... More
The browning of white adipose tissue and body weight loss in primary hyperparathyroidism.
BACKGROUND: Parathyroid hormone related protein (PTHrP) triggers white adipose tissue (WAT) browning and cachexia in lung cancer mouse models. It remains unknown whether excessive PTH secretion affects WAT browning and to what extent it contributes to body weight change in primary hyperparathyroidism (PHPT). METHODS: Using the adeno-associated virus injection, Pth ... More
Effects of paternal obesity on growth and adiposity of male rat offspring.
AuthorsLecomte V,Maloney CA,Wang KW,Morris MJ
JournalAmerican journal of physiology. Endocrinology and metabolism
PubMed ID27965204
Serotype specific epitopes identified by neutralizing antibodies underpin immunogenic differences in Enterovirus B.
Echovirus 30 (E30), a serotype of Enterovirus B (EV-B), recently emerged as a major causative agent of aseptic meningitis worldwide. E30 is particularly devastating in the neonatal population and currently no vaccine or antiviral therapy is available. Here we characterize two highly potent E30-specific monoclonal antibodies, 6C5 and 4B10, which ... More
linc00968 inhibits the tumorigenesis and metastasis of lung adenocarcinoma via serving as a ceRNA against miR-9-5p and increasing CPEB3.
AuthorsTang H,Han X,Feng Y,Hao Y
JournalAging
PubMed ID33159015
Increasing evidence confirms that long noncoding RNAs (lncRNAs) exert vital functions in multiple biological process among malignant cancers. In the current study, we uncovered that linc00968 was downregulated in lung adenocarcinoma (LUAD). Furthermore, the low level of linc00968 was correlated with worse prognosis in patients with LUAD. Upregulation of linc00968 ... More
IRX3 Overexpression Enhances Ucp1 Expression In Vivo.
OBJECTIVE: The Iroquois homeobox 3 (IRX3) gene was recently reported to be a functional downstream target of a common polymorphism in the FTO gene, which encodes an obesity-associated protein; however, the role of IRX3 in energy expenditure remains unclear. Studies have revealed that the overexpression of a dominantânegative form of ... More
Genetic variations of DICKKOPF family genes might not be associated with gastric cancer susceptibility.
AuthorsWu J,Zhang J,Zhan Z,Cao Q,Li Z
JournalBMC gastroenterology
PubMed ID27457487
BACKGROUND: Recent studies have implicated that members of the DICKKOPF (DKK) were causally involved in large number of human cancers. This study was designed to investigate the relationship between the genetic variations of DKK family genes and the risk of gastric cancer (GC). METHODS: Six SNPs (single nucleotide polymorphisms) of ... More
Mutation Survey of Candidate Genes and Genotype-Phenotype Analysis in 20 Southeastern Chinese Patients with Axenfeld-Rieger Syndrome.
AuthorsWang X,Liu X,Huang L,Fang S,Jia X,Xiao X,Li S,Guo X
JournalCurrent eye research
PubMed ID29939776
Exosomal LncRNA LBX1-AS1 Derived From RBPJ Overexpressed-Macrophages Inhibits Oral Squamous Cell Carcinoma Progress via miR-182-5p/FOXO3.
AuthorsAi Y,Wei H,Wu S,Tang Z,Li X,Zou C
JournalFrontiers in oncology
PubMed ID33816238
OBJECTIVES: Macrophage-derived exosomes (MÏ-Exos) are involved in tumor onset, progression, and metastasis, but their regulation in oral squamous cell carcinoma (OSCC) is not fully understood. RBPJ is implicated in macrophage activation and plasticity. In this study, we assessed the role of MÏ-Exos with RBPJ overexpression (RBPJ-OE MÏ-Exos) in OSCC. MATERIALS ... More
A Community-Based Management of COVID-19 in a Mobile Container Unit.
AuthorsPetrova E,Farinholt T,Joshi TP,Moreno H,Al Mohajer M,Patel SM,Petrosino J,Anandasabapathy S
JournalVaccines
PubMed ID34835293
Vaccine uptake is a multifactor measure of successful immunization outcomes that includes access to healthcare and vaccine hesitancy for both healthcare workers and communities. The present coronavirus disease (COVID-19) pandemic has highlighted the need for novel strategies to expand vaccine coverage in underserved regions. Mobile clinics hold the promise of ... More
Delayed Thrombin Generation Is Associated with Minor Bleedings in Venous Thromboembolism Patients on Rivaroxaban: Usefulness of Calibrated Automated Thrombography.
AuthorsZalewski J,Stepien K,Nowak K,Caus S,Butenas S,Undas A
JournalJournal of clinical medicine
PubMed ID32605001
Bleeding is the most feared and difficult to predict adverse event of anticoagulation. We sought to investigate whether calibrated automated thrombography (CAT) parameters are associated with minor bleeding (MB) in anticoagulated patients following venous thromboembolism (VTE). Enrolled were 132 patients on rivaroxaban, 145 on vitamin K antagonists (VKA) and 31 ... More
CircRNA CDR1as promotes hepatoblastoma proliferation and stemness by acting as a miR-7-5p sponge to upregulate KLF4 expression.
AuthorsChen L,Shi J,Wu Y,Qiu R,Zeng L,Lou L,Su J,Liao M,Deng X
JournalAging
PubMed ID33052880
Hepatoblastoma (HB) is a malignant embryonal tumor of the liver that consists of heterogenous populations of stem/progenitor cells. Although circular RNAs (circRNAs) play an essential role in tumor development, the effects of circRNA on the proliferation of HB cells, especially cancer stem cells (CSCs), remain unclear. We found that the ... More
Feminizing Adrenocortical Carcinoma: The Source of Estrogen Production and the Role of Adrenal-Gonadal Dedifferentiation.
JournalThe Journal of clinical endocrinology and metabolism
PubMed ID30053001
Upregulated TRAIL and Reduced DcR2 Mediate Apoptosis of Decidual PMN-MDSC in Unexplained Recurrent Pregnancy Loss.
AuthorsLi C,Zhang X,Kang X,Chen C,Guo F,Wang Q,Zhao A
JournalFrontiers in immunology
PubMed ID32695113
Myeloid-derived suppressor cells (MDSC), especially polymorphonuclear MDSC (PMN-MDSC), accumulate in maternal-fetal interface during pregnancy and are involved in the maintenance of immune tolerance. Decreased PMN-MDSC is associated with pregnancy complications such as unexplained recurrent pregnancy loss (URPL). In the present study we showed decreased PMN-MDSC in the URPL group compared ... More
Effect of autologous NK cell immunotherapy on advanced lung adenocarcinoma with EGFR mutations.
AuthorsHong G,Chen X,Sun X,Zhou M,Liu B,Li Z,Yu Z,Gao W,Liu T
JournalPrecision clinical medicine
PubMed ID35693880
This study investigated the efficiency of natural killer (NK) cell immunotherapy on non-small cell lung cancer with and without EGFR mutations in order to evaluate the response rate (RR) and progression-free survival (PFS). Among the 48 patients recruited, 24 were clinically confirmed to be EGFR mutation positive. The study group ... More
Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses.
The emergence and spread of Zika virus (ZIKV) presented a challenge to the diagnosis of ZIKV infections in areas with transmission of dengue (DENV) and chikungunya (CHIKV) viruses. To facilitate detection of ZIKV infections, and differentiate these infections from DENV and CHIKV, we developed the Trioplex real-time RT-PCR assay (Trioplex ... More
IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk.
BACKGROUND: IRX3 was recently reported as the effector of the FTO variants. We aimed to test IRX3's roles in the browning program and to evaluate the association between the genetic variants in IRX3 and human obesity. METHODS: IRX3 expression was examined in beige adipocytes in human and mouse models, and ... More
circ5912 suppresses cancer progression via inducing MET in bladder cancer.
AuthorsSu Y,Du Z,Zhong G,Ya Y,Bi J,Shi J,Chen L,Dong W,Lin T
JournalAging
PubMed ID31808751
Background: Increasing evidence suggests that circular RNAs play a key role in regulating bladder cancer progression. However, this remains to be fully elucidated. Results: In this study, we reanalyzed our previous RNA sequence, and circ5912 was found to downregulate significantly in bladder cancer tissues compared with normal control. Expression of ... More
PSMD7 downregulation suppresses lung cancer progression by regulating the p53 pathway.
AuthorsXu X,Xuan X,Zhang J,Xu H,Yang X,Zhang L,Zhao Y,Xu H,Li D
JournalJournal of Cancer
PubMed ID34234864
Lung cancer is the second most common cancer in both men and women. The deubiquitinase PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. Currently, PSMD7 expression and its roles in the progression of lung cancer remain largely unknown. ... More
miR-502-mediated histone methyltransferase SET8 expression is associated with clear cell renal cell carcinoma risk.
AuthorsZhang S,Guo Z,Xu J,Wang J,Zhang J,Cui L,Zhang H,Liu Y,Bai Y
JournalOncology letters
PubMed ID29250163
Genetic variants may affect the interactions between microRNAs (miRNAs/miRs) and their target genes by modulating their binding affinity or by creating, or destroying a miRNA-binding site. SET domain containing (lysine methyltransferase) 8 (SET8) is the sole lysine methyltransferase that catalyzes the monomethylation of histone H4 lysine 20, and is associated ... More
Hsa_circ_0002577 promotes endometrial carcinoma progression via regulating miR-197/CTNND1 axis and activating Wnt/β-catenin pathway.
AuthorsShen Q,He T,Yuan H
JournalCell cycle (Georgetown, Tex.)
PubMed ID31081718
Circular RNA (circRNA) is involved in a wide range of life processes including tumorigenesis. However, the molecular mechanisms of circRNA in endometrial carcinoma (EC) carcinogenesis remain unclear. In the present study, we aimed to investigate the potential modulation of hsa_circ_0002577 on EC progression. Here, we showed that hsa_circ_0002577 expression was ... More
STAMBP promotes lung adenocarcinoma metastasis by regulating the EGFR/MAPK signaling pathway.
AuthorsXu H,Yang X,Xuan X,Wu D,Zhang J,Xu X,Zhao Y,Ma C,Li D
JournalNeoplasia (New York, N.Y.)
PubMed ID34102455
Tumor metastasis is a leading cause of death in lung adenocarcinoma (LUAD) patients, but the molecular events that regulate metastasis have not been completely elucidated. STAMBP is a deubiquitinating enzyme of the Jab1/MPN metalloenzyme family that regulates the stability of substrates in cells by specifically removing ubiquitin molecules. We found ... More
Association Analysis of Variants of DSCAM and BACE2 With Hirschsprung Disease Susceptibility in Han Chinese and Functional Evaluation in Zebrafish.
JournalFrontiers in cell and developmental biology
PubMed ID34136475
Hirschsprung disease (HSCR) has a higher incidence in children with Down syndrome (DS), which makes trisomy 21 a predisposing factor to HSCR. DSCAM and BACE2 are close together on the HSCR-associated critical region of chromosome 21. Common variants of DSCAM and rare variants of BACE2 were implicated to be associated ... More
Upregulation of deubiquitinase PSMD14 in lung adenocarcinoma (LUAD) and its prognostic significance.
AuthorsZhang L,Xu H,Ma C,Zhang J,Zhao Y,Yang X,Wang S,Li D
JournalJournal of Cancer
PubMed ID32226511
PSMD14 is a 19S-proteasome-associated deubiquitinating enzyme that facilitates protein degradation by the 20S proteasome core particle. Although accumulating evidence indicates that PSMD14 has emerged as a critical oncogenic factor by promoting tumor growth, the expression and function of PSMD14 in non-small cell lung cancer (NSCLC) remain largely unknown. In this ... More
YY1 regulation by miR-124-3p promotes Th17 cell pathogenicity through interaction with T-bet in rheumatoid arthritis.
Th17 cells are involved in rheumatoid arthritis (RA) pathogenesis. Our previous studies have revealed that transcription factor Yin Yang 1 (YY1) plays an important role in the pathogenic mechanisms of RA. However, whether YY1 has any role in Th17 cell pathogenicity and what molecular regulatory mechanism is involved remain poorly ... More
Hypoxia-inducible factor 1 mediates intermittent hypoxia-induced migration of human breast cancer MDA-MB-231 cells.
AuthorsLiu L,Liu W,Wang L,Zhu T,Zhong J,Xie N
JournalOncology letters
PubMed ID29250173
Metastasis is the major cause of triple-negative breast cancer (TNBC)-associated mortality. Hypoxia promotes cancer cell migration and remote metastasis, which occur with hypoxia inducible factor 1α (HIF-1α) stabilization and vimentin upregulation. However, the evolutionary dynamics that link the changes in HIF-1α and vimentin levels under hypoxic conditions are not well ... More
Decreased MiR-485-5p Contributes to Inflammatory Pain Through Post-Transcriptional Upregulation of ASIC1 in Rat Dorsal Root Ganglion.
AuthorsXu M,Wu R,Zhang L,Zhu HY,Xu GY,Qian W,Zhang PA
JournalJournal of pain research
PubMed ID33239909
BACKGROUND: Inflammatory pain is the most common type of pain treated clinically. However, the currently available treatments for inflammatory pain have limited effects and can cause severe side effects. The aim of this study is to describe the effect of miRNA-485-5p on osteoarthritis-related inflammatory pain. METHODS: Paw withdrawal threshold (PWT) ... More
Hsa_circ_0000069 Knockdown Inhibits Tumorigenesis and Exosomes with Downregulated hsa_circ_0000069 Suppress Malignant Transformation via Inhibition of STIL in Pancreatic Cancer.
AuthorsYe Z,Zhu Z,Xie J,Feng Z,Li Y,Xu X,Li W,Chen W
JournalInternational journal of nanomedicine
PubMed ID33324055
BACKGROUND: Circular RNAs (circRNAs) play an important role in the tumorigenesis of pancreatic cancer. However, the expression profiles and roles of circRNAs in pancreatic cancer remain largely unknown. METHODS: To identify differentially expressed circRNAs (DEcircRNAs) between pancreatic cancer and matched normal tissues, bioinformatics analysis was performed. Hsa_circ_0000069 was identified by ... More
Comparative analysis of the accelerated aged seed transcriptome profiles of two maize chromosome segment substitution lines.
AuthorsLi L,Wang F,Li X,Peng Y,Zhang H,Hey S,Wang G,Wang J,Gu R
JournalPloS one
PubMed ID31710606
Seed longevity is one of the most essential characteristics of seed quality. Two chromosome segment substitution lines, I178 and X178, which show significant differences in seed longevity, were subjected to transcriptome sequencing before and after five days of accelerated aging (AA) treatments. Compared to the non-aging treatment, 286 and 220 ... More
Deubiquitinase UCHL5 is elevated and associated with a poor clinical outcome in lung adenocarcinoma (LUAD).
Lung cancer is one of the most common malignant tumors in the world, with a high rate of malignancy and mortality. Seeking new biomarkers and potential drug targets is urgent for effective treatment of the disease. Deubiquitinase UCHL5/UCH37, as an important component of the 26S proteasome, plays critical roles in ... More
Chimeric antigen receptor T cells derived from CD7 nanobody exhibit robust antitumor potential against CD7-positive malignancies.
The great success of chimeric antigen receptor T (CAR-T)-cell therapy in B-cell malignancies has significantly promoted its rapid expansion to other targets and indications, including T-cell malignancies and acute myeloid leukemia. However, owing to the life-threatening T-cell hypoplasia caused by CD7-CAR-T cells specific cytotoxic against normal T cells, as well ... More
Toilets dominate environmental detection of severe acute respiratory syndrome coronavirus 2 in a hospital.
Respiratory and fecal aerosols play confirmed and suspected roles, respectively, in transmitting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). An extensive environmental sampling campaign of both toilet and non-toilet environments was performed in a dedicated hospital building for patients with coronavirus disease 2019 (COVID-19), and the associated environmental factors were ... More
The Effects of Neurokinin-1 Receptor Antagonist in an Experimental Autoimmune Cystitis Model Resembling Bladder Pain Syndrome/Interstitial Cystitis.
AuthorsLiu BK,Jin XW,Lu HZ,Zhang X,Zhao ZH,Shao Y
JournalInflammation
PubMed ID30196377
Whole-Tumor Histogram and Texture Imaging Features on Magnetic Resonance Imaging Combined With Epstein-Barr Virus Status to Predict Disease Progression in Patients With Nasopharyngeal Carcinoma.
AuthorsLi Q,Wang T,Huang Y,Li Q,Liu P,Grimm R,Fu C,Zhang Y,Gu Y
JournalFrontiers in oncology
PubMed ID33767984
Purpose: We aimed to investigate whether EpsteinâBarr virus (EBV) could produce differences on MRI by examining the histogram and texture imaging features. We also sought to determine the predictive value of pretreatment MRI texture analyses incorporating with EBV status for disease progression (PD) in patients with primary nasopharyngeal carcinoma (NPC). ... More
Development and Potential Usefulness of the COVID-19 Ag Respi-Strip Diagnostic Assay in a Pandemic Context.
AuthorsMertens P,De Vos N,Martiny D,Jassoy C,Mirazimi A,Cuypers L,Van den Wijngaert S,Monteil V,Melin P,Stoffels K,Yin N,Mileto D,Delaunoy S,Magein H,Lagrou K,Bouzet J,Serrano G,Wautier M,Leclipteux T,Van Ranst M,Vandenberg O,LHUB-ULB SARS-CoV-2 Working Diagnostic Group
JournalFrontiers in medicine
PubMed ID32574326
Introduction: COVID-19 Ag Respi-Strip, an immunochromatographic (ICT) assay for the rapid detection of SARS-CoV-2 antigen on nasopharyngeal specimen, has been developed to identify positive COVID-19 patients allowing prompt clinical and quarantine decisions. In this original research article, we describe the conception, the analytical and clinical performances as well as the ... More
A Novel mRNA-miRNA Regulatory Sub-Network Associated With Prognosis of Metastatic Clear Cell Renal Cell Carcinoma.
AuthorsYang T,Miao X,Bai Z,Tu J,Shen S,Niu H,Xia W,Wang J,Zhang Y
JournalFrontiers in oncology
PubMed ID33542901
BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is a urinary disease with high incidence. The high incidence of metastasis is the leading cause of death in patients with ccRCC. This study was aimed to identify the gene signatures during the metastasis of ccRCC. METHODS: Two datasets, including one gene expression ... More
Development and Clinical Application of a Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for SARS-CoV-2 Infection.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak urgently necessitates sensitive and convenient COVID-19 diagnostics for the containment and timely treatment of patients. We aimed to develop and validate a novel reverse transcriptionâloop-mediated isothermal amplification (RT-LAMP) assay to detect SARS-CoV-2. Patients with suspected COVID-19 and close contacts were recruited ... More
Synergism between the phosphatidylinositol 3-kinase p110β isoform inhibitor AZD6482 and the mixed lineage kinase 3 inhibitor URMC-099 on the blockade of glioblastoma cell motility and focal adhesion formation.
BACKGROUND: Glioblastoma multiforme, the most aggressive and malignant primary brain tumor, is characterized by rapid growth and extensive infiltration to neighboring normal brain parenchyma. Our previous studies delineated a crosstalk between PI3K/Akt and JNK signaling pathways, and a moderate anti-glioblastoma synergism caused by the combined inhibition of PI3K p110β (PI3Kβ) ... More
Development and Clinical Validation of a 90-Gene Expression Assay for Identifying Tumor Tissue Origin.
JournalSmall (Weinheim an der Bergstrasse, Germany)
PubMed ID32578378
The ongoing global novel coronavirus pneumonia COVIDâ19 outbreak has engendered numerous cases of infection and death. COVIDâ19 diagnosis relies upon nucleic acid detection; however, currently recommended methods exhibit high falseânegative rates and are unable to identify other respiratory virus infections, thereby resulting in patient misdiagnosis and impeding epidemic containment. Combining ... More
Development of a Multiplex Real-Time PCR Assay for the Newborn Screening of SCID, SMA, and XLA.
JournalInternational journal of neonatal screening
PubMed ID33072998
Numerous studies have shown evidence supporting the benefits of universal newborn screening for primary immunodeficiencies (PID) and for Spinal Muscular Atrophy (SMA). We have developed a four-plex, real-time PCR assay to screen for Severe Combined Immune Deficiencies (SCID), X-linked agammaglobulinemia (XLA), and SMA in DNA extracted from a single 3.2 ... More
Diagnostic performances of common nucleic acid tests for SARS-CoV-2 in hospitals and clinics: a systematic review and meta-analysis.
AuthorsAu WY,Cheung PPH
JournalThe Lancet. Microbe
PubMed ID34661181
BACKGROUND: An optimised standard experimental setup across different hospitals is urgently needed to ensure consistency in nucleic acid test results for SARS-CoV-2 detection. A standard comparison across different nucleic acid tests and their optimal experimental setups is not present. We assessed the performance of three common nucleic acid tests, namely ... More
Low HOXC10 expression in liver cancer regulates proliferation via a mechanism involving miR-221 and the MAPK signaling pathway.
Homeodomain-containing gene 10 (HOXC10) is associated with the progression of a variety of different types of human cancer; however, the role of HOXC10 in liver cancer is not completely understood. The present study aimed to investigate the mechanisms underlying the effects of HOXC10 on liver cancer tumorigenesis. Quantitative PCR and ... More
The roles of AMPK-mediated autophagy and mitochondrial autophagy in a mouse model of imiquimod-induced psoriasis.
Background: Psoriasis is a systemic inflammatory disease characterized by epidermal hyperplasia and skin inflammatory infiltrates. Inactivation of AMPK has been shown to decrease autophagy, thereby inhibiting elimination of inflammatory factors and harmful substances, and aggravating psoriasis. However, the molecular mechanism through which AMPK affects psoriasis remains to be further explored. ... More
OTUD5 cooperates with TRIM25 in transcriptional regulation and tumor progression via deubiquitination activity.
Oncogenic processes exert their greatest effect by targeting regulators of cell proliferation. Studying the mechanism underlying growth augmentation is expected to improve clinical therapies. The ovarian tumor (OTU) subfamily deubiquitinases have been implicated in the regulation of critical cell-signaling cascades, but most OTUs functions remain to be investigated. Through an ... More
High TNFSF13B expression as a predictor of poor prognosis in adrenocortical carcinoma.
AuthorsMao Y,Alimu P,Wang C,Ma W,Zhuo R,Sun F
JournalTranslational andrology and urology
PubMed ID34532252
BACKGROUND: Adrenocortical carcinoma (ACC) is an extremely rare malignant tumor with poor prognosis. Existing treatment options have limited effects, and new therapeutic targets urgently need to be discovered. TNFSF13B has been reported to be associated with the prognosis of clear cell renal cell carcinoma, but it has not been studied ... More
Implementation of an Extraction-Free COVID Real-Time PCR Workflow in a Pediatric Hospital Setting.
BACKGROUND: This study outlines the development, implementation, and impact of a laboratory-developed, extraction-free real-time PCR assay as the primary diagnostic test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a pediatric hospital. METHODS: Clinical specimens from both upper and lower respiratory tract sources were validated, including nasopharyngeal aspirates, nasopharyngeal ... More
CircRNA WHSC1 promotes non-small cell lung cancer progression via sponging microRNA-296-3p and up-regulating expression of AKT serine/threonine kinase 3.
AuthorsShi F,Yang Q,Shen D,Chen J
JournalJournal of clinical laboratory analysis
PubMed ID34313353
BACKGROUND: Lung cancer is the most commonly diagnosed cancer and leading cause of cancer death, with 80%â85% of nonâsmall cell lung cancer (NSCLC). Circular RNAs (circRNAs) have been shown to be promising early diagnostic and therapeutic molecular biomarkers for NSCLC. However, biological role and regulatory mechanism of circRNA WHSC1 (circWHSC1) ... More
Deubiquitinase PSMD7 regulates cell fate and is associated with disease progression in breast cancer.
AuthorsZhao Y,Yang X,Xu X,Zhang J,Zhang L,Xu H,Miao Z,Li D,Wang S
JournalAmerican journal of translational research
PubMed ID33042429
Breast cancer is the most common malignant tumor and the leading cause of cancer-related death in women. The ubiquitin-proteasome system regulates the stability of most proteins controlling various biological processes in human cells. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins ... More
Hydrazinocurcumin and 5-fluorouracil enhance apoptosis and restrain tumorigenicity of HepG2 cells via disrupting the PTEN-mediated PI3K/Akt signaling pathway.
AuthorsZhou Y,Zhang M,Zhang Z,Jia Y,Zhang C,Peng L
Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.
AuthorsPadgett LR,Kennington LA,Ahls CL,Samarasinghe DK,Tu YP,Wallander ML,Cooper SD,Elliott JS,Rains D
JournalPloS one
PubMed ID33852576
BACKGROUND: In order to identify an inexpensive yet highly stable SARS-CoV-2 collection device as an alternative to foam swabs stored in transport media, both contrived (âsurrogateâ) CoV-positive and patient-collected spun polyester swabs stored in dry tubes were evaluated for time- and temperature-stability using qPCR. METHODS: Surrogate specimens were prepared by ... More
SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu.
AuthorsPan K,Huang X,Jia X
JournalOncology letters
PubMed ID33968201
SPARC is a secreted glycoprotein that plays a complex and multifaceted role in tumour formation and progression. However, whether SPARC is an oncogene or a tumour suppressor is still unclear. Moreover, SPARC demonstrates potential in clinical pancreatic adenocarcinoma (PAAD) treatment, although it has been identified as an oncogene in some ... More
Luteolin transforms the polarity of bone marrow-derived macrophages to regulate the cytokine storm.
AuthorsWang S,Xu S,Zhou J,Zhang L,Mao X,Yao X,Liu C
JournalJournal of inflammation (London, England)
PubMed ID34059076
BACKGROUND: Macrophages are indispensable regulators of inflammatory responses. Macrophage polarisation and their secreted inflammatory factors have an association with the outcome of inflammation. Luteolin, a flavonoid abundant in plants, has anti-inflammatory activity, but whether luteolin can manipulate M1/M2 polarisation of bone marrow-derived macrophages (BMDMs) to suppress inflammation is still unclear. ... More
COX4I2 is a novel biomarker of blood supply in adrenal tumors.
AuthorsMao Y,Ma W,Zhuo R,Ye L,Xu D,Wang W,Ning G,Sun F
JournalTranslational andrology and urology
PubMed ID34430392
BACKGROUND: Previous study has been reported that COX4I2 expression level demonstrated a positive correlation with microvessel density in pheochromocytomas (PCC) samples, suggesting that the expression of COX4I2 maybe related to blood supply level in other adrenal tumors as well. The aim of this study is to clarify the correlation of ... More
Identification of prognostic values defined by copy number variation, mRNA and protein expression of LANCL2 and EGFR in glioblastoma patients.
BACKGROUND: Epidermal growth factor receptor (EGFR) and lanthionine synthetase C-like 2 (LanCL2) genes locate in the same amplicon, and co-amplification of EGFR and LANCL2 is frequent in glioblastoma. However, the prognostic value of LANCL2 and EGFR co-amplification, and their mRNA and protein expression in glioblastoma remain unclear yet. METHODS: This ... More
Osteocalcin ameliorates cognitive dysfunctions in a mouse model of Alzheimer's Disease by reducing amyloid β burden and upregulating glycolysis in neuroglia.
Alzheimerâs disease (AD) is the most common neurodegenerative disease characterized by the accumulation of amyloid β peptides (Aβ) and impaired glucose metabolism in the brain. Osteocalcin (OCN), an osteoblast-derived protein, has been shown to modulate brain functions but whether it has any effect on AD is undetermined. In this study, ... More
Identification of Grade-associated MicroRNAs in Brainstem Gliomas Based on Microarray Data.
Gliomas arising in the brainstem are rare tumours that are difficult to surgically resect, and the microRNAs (miRNAs) and signalling pathways associated with brainstem gliomas (BSGs) are largely unknown. To identify grade-associated miRNAs in BSGs, a microarray analysis of 10 low-grade and 15 high-grade BSGs was performed in this study. ... More
Elevated expression of the RNA-binding protein IGF2BP1 enhances the mRNA stability of INHBA to promote the invasion and migration of esophageal squamous cancer cells.
BACKGROUND: The mechanisms underlying the occurrence and development of esophageal squamous cell carcinoma (ESCC) remains to be elucidated. The present study aims to investigate the roles and implications of IGF2BP1 overexpression in ESCC. METHODS: IGF2BP1 protein expression in ESCC samples was assessed by immunohistochemistry (IHC), and the mRNA abundance of ... More
Effect of ORF7 of SARS-CoV-2 on the Chemotaxis of Monocytes and Neutrophils In Vitro.
Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently the most significant public health threat worldwide. Patients with severe COVID-19 usually have pneumonia concomitant with local inflammation and sometimes a cytokine storm. Specific components of the SARS-CoV-2 virus trigger lung inflammation, and recruitment ... More
Intracellular Lipid Accumulation Drives the Differentiation of Decidual Polymorphonuclear Myeloid-Derived Suppressor Cells via Arachidonic Acid Metabolism.
Decidual polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) are essential to immune tolerance during pregnancy. A reduction in the number of these cells is associated with unexplained recurrent pregnancy loss (URPL). In our previous study, we reported that PMN-MDSCs are a group of mature neutrophils that are activated by the decidua microenvironment. ... More
Identification of a novel immune-related transcriptional regulatory network in sarcopenia.
BACKGROUND: Sarcopenia is highly prevalent in elderly individuals and has a significant adverse effect on their physical health and quality of life, but the mechanisms remain unclear. Studies have indicated that transcription factors (TFs) and the immune microenvironment play a vital role in skeletal muscle atrophy. METHODS: RNA-seq data of ... More
DNMT3a-dermatopontin axis suppresses breast cancer malignancy via inactivating YAP.
AuthorsYe D,Wang Y,Deng X,Zhou X,Liu D,Zhou B,Zheng W,Wang X,Fang L
JournalCell death & disease
PubMed ID36774339
Breast cancer (BC) is the most common malignant tumor in women worldwide, and its recurrence and metastasis negatively affect patient prognosis. However, the mechanisms underlying its tumorigenesis and progression remain unclear. Recently, the influence of dermatopontin (DPT), which is an extracellular matrix protein, has been proposed in the development of ... More
REV-ERB is essential in cardiac fibroblasts homeostasis.
REV-ERB agonists have shown antifibrotic effects in the heart and other organs. The function of REV-ERB in the cardiac fibroblasts remains unstudied. Here, we characterize the functional difference of REV-ERB in mouse embryonic fibroblasts and cardiac fibroblasts using genetic deletion of REV-ERBα and à in vitro. We show that REV-ERB ... More
Decreased Expression of KLF4 Leading to Functional Deficit in Pediatric Patients with Intestinal Failure and Potential Therapeutic Strategy Using Decanoic Acid.
AuthorsYan J,Zhao Y,Jiang L,Wang Y,Cai W
JournalNutrients
PubMed ID37375564
Pediatric intestinal failure (IF) is the reduction in gut function to below the minimum necessary for the absorption of macronutrients and/or water and electrolytes, such that intravenous supplementation is required to maintain health and/or growth. The overall goal in treating IF is to achieve intestinal adaptation; however, the underlying mechanisms ... More
Exosomal circRNA BTG2 derived from RBP-J overexpressed-macrophages inhibits glioma progression via miR-25-3p/PTEN.
AuthorsShi L,Cao Y,Yuan W,Guo J,Sun G
JournalCell death & disease
PubMed ID35643814
Macrophage-derived exosomes (MÏ-Exos) are involved in tumor progression, but its role in glioma is not fully understood. RBP-J is related to macrophage activation. In this study, we assess the role of exosomes derived from RBP-J-overexpressed macrophages (RBP-J OE MÏ-Exos) in glioma. The circular RNA (circRNA) profiles in RBP-J OE MÏ-Exos ... More
Common variation of the NSD1 gene is associated with susceptibility to Hirschsprung's disease in Chinese Han population.
Age-related osteoporosis is characterized by the accumulation of senescent osteoblastic cells in bone microenvironment and significantly reduced osteogenic differentiation. Clearing of the senescent cells is helpful to improve bone formation in aged mice. Bone morphogenetic protein 9 (BMP9), a multifunctional protein produced and secreted by liver, was reported to improve ... More
Optimized protocols for chromatin immunoprecipitation of exogenously expressed epitope-tagged proteins.
AuthorsFang W,Liao C,Zhang Q
JournalSTAR protocols
PubMed ID36853721
Chromatin immunoprecipitation (ChIP) assay is widely used for investigating the interaction between DNA and DNA-binding proteins such as transcription factors, co-factors, or chromatin-associated proteins. However, a successful ChIP assay largely depends on the quality of a ChIP-grade primary antibody. In cases where specific antibodies are unavailable or with low binding ... More
A Novel Blood-Based Panel of Methylated DNA and Protein Markers for Detection of Early-Stage Hepatocellular Carcinoma.
JournalClinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association
PubMed ID32889146
MiR-1224 Acts as a Prognostic Biomarker and Inhibits the Progression of Gastric Cancer by Targeting SATB1.
AuthorsHan GD,Sun Y,Hui HX,Tao MY,Liu YQ,Zhu J
JournalFrontiers in oncology
PubMed ID34604093
OBJECTIVE: MiR-1224 has been reported to exhibit abnormal expression in several tumors. However, the expressing pattern and roles of miR-1224 in gastric cancer (GC) remain unclear. Our current research aimed to explore the potential involvement of miR-1224 in the GC progression. MATERIALS AND METHODS: The expression of miR-1224 was examined ... More
Detection of Torquetenovirus and Redondovirus DNA in Saliva Samples from SARS-CoV-2-Positive and -Negative Subjects.
Objectives: Torquetenovirus (TTV) and Redondovirus (ReDoV) are the most prevalent viruses found in the human respiratory virome in viral metagenomics studies. A large-scale epidemiological study was performed to investigate their prevalence and loads in saliva samples according to SARS-CoV-2 status. Methods: Saliva samples from 448 individuals (73% SARS-CoV-2 negative and ... More
A Common Variant of ASAP1 Is Associated with Tuberculosis Susceptibility in the Han Chinese Population.
AuthorsChen C,Zhao Q,Shao Y,Li Y,Song H,Li G,Zhu L,Lu W,Xu B
JournalDisease markers
PubMed ID31089398
BACKGROUND: ASAP1 (also known as AMAP1 or DDEF1) encodes an Arf GTPase-activating protein (Arf GAP), a multifunctional scaffold protein that induces hydrolysis of GTP bound to the ADP-ribosylation factor (Arf) family GTP-binding proteins. Reduction of ASAP1 expression in vitro was related to suppression of cell migration and invasiveness. The genetic ... More
Bioinspired tumor-homing nanoplatform for co-delivery of paclitaxel and siRNA-E7 to HPV-related cervical malignancies for synergistic therapy.
AuthorsXu C,Liu W,Hu Y,Li W,Di W
JournalTheranostics
PubMed ID32194871
Because of the complexity of cancer, a combination of chemotherapy and gene therapy is an emerging treatment modality. To realize the full potential of this strategy, a smart, highly biocompatible nanosystem that enables the precise co-delivery of small-molecule anticancer drugs and small interfering RNA (siRNA) is urgently needed. This study ... More
The non-telomeric evolutionary trajectory of TRF2 in zebrafish reveals its specific roles in neurodevelopment and aging.
The shelterin protein complex is required for telomere protection in various eukaryotic organisms. In mammals, the shelterin subunit TRF2 is specialized in preventing ATM activation at telomeres and chromosome end fusion in somatic cells. Here, we demonstrate that the zebrafish ortholog of TRF2 (encoded by the terfa gene) is protecting ... More
Valbenazine promotes body growth via growth hormone signaling during zebrafish embryonic development.
AuthorsSu Z,Dai Z,Qin F,Zhang H,Zheng M,Zhu Y,Tong Z,Song W,Li X
JournalToxicology and applied pharmacology
PubMed ID37648088
The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice.
Thyrotropin (TSH) is a modulator of glucose metabolism by binding to its receptor on pancreatic cells. We used thyrotropin receptor (TSHR) knockout mice (Tshr(â/â)) as a model of TSH deletion to study its function in pancreatic β cells. Tshr(â/â) mice had a similar body weight at birth compared with Tshr(+/+) ... More
Identification of Biomarkers for Predicting Ovarian Reserve of Primordial Follicle via Transcriptomic Analysis.
Ovarian reserve (OR) is mainly determined by the number of primordial follicles in the ovary and continuously depleted until ovarian senescence. With the development of assisted reproductive technology such as ovarian tissue cryopreservation and autotransplantation, growing demand has arisen for objective assessment of OR at the histological level. However, no ... More
SNORA56-mediated pseudouridylation of 28 S rRNA inhibits ferroptosis and promotes colorectal cancer proliferation by enhancing GCLC translation.
JournalJournal of experimental & clinical cancer research : CR
PubMed ID38049865
BACKGROUND: Colorectal cancer (CRC) is one of the most common malignancies and is characterized by reprogrammed metabolism. Ferroptosis, a programmed cell death dependent on iron, has emerged as a promising strategy for CRC treatment. Although small nucleolar RNAs are extensively involved in carcinogenesis, it is unclear if they regulate ferroptosis ... More
A novel insight into the key gene signature associated with the immune landscape in the progression of sarcopenia.
AuthorsShen ZL,Chen WH,Liu Z,Yu DY,Chen WZ,Zang WF,Zhang P,Yan XL,Yu Z
JournalExperimental gerontology
PubMed ID37343810
Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma.
The aim of the present study was to evaluate the expression of TRAF2- and NCK-interacting kinase (TNIK) and the levels of the active form of TNIK, phosphorylated (p)-TNIK, in papillary thyroid carcinoma (PTC), and to identify and compare the levels of TNIK and p-TNIK among PTC, benign thyroid tumors and ... More
An Inflammatory Response-Related Gene Signature Can Predict the Prognosis and Impact the Immune Status of Lung Adenocarcinoma.
AuthorsShi Y,Zhao Y,Wang Y
JournalCancers
PubMed ID36497225
SIMPLE SUMMARY: Lung adenocarcinoma (LUAD) is a highly prevalent and deadly global malignancy with an annual increase in its incidence. The association of inflammation with tumor genesis and development has been of interest in recent years. However, the association of inflammatory response-related genes (IRGs) with LUAD remains unclear. In this ... More
Point-Of-Care Ultra-Portable Single-Molecule Bioassays for One-Health.