Need help deciding what product to use with the Ion Torrent™ Sequencing System? Check out our helpful Product Selection Guides for Ion S5™ and Ion S5 XL™ Systems and Ion PGM™ System.
Ion Torrent™

Ion AmpliSeq™ Library Kit 2.0

Catalog number:  4480441

Related applications:

DNA Sequencing

| Ion Torrent™ Next-Generation Sequencing

Back to top

Description

The Ion AmpliSeq™ Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ panels.

Scalable Multiplex PCR Reactions
The Ion AmpliSeq 2.0 technology enables scalable multiplex PCR reactions from 12- to 24,000-plex in a single well using just 10 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Ion AmpliSeq™ Custom Primer Pools are designed via the Ion AmpliSeq™ Designer Tool, available at www.ampliseq.com.

Barcoded Library Preparation
The Ion AmpliSeq Library Kit 2.0 includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1-96 kits or IonCode™ Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and Flexible Protocol
The Ion AmpliSeq Library Kit 2.0 uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The library preparation uses very small quantities of starting material per PCR. The resulting DNA libraries are ready for downstream template preparation using the automated Ion Chef™ System (which also provides the option of automated library preparation using the Ion AmpliSeq™ Kit for Chef DL8) or the Ion OneTouch™ 2 System followed by sequencing. The intuitive Torrent Suite™ Software and optional Ion Reporter™ Software enables automation of data analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

High-throughput Compatibility: High-throughput Compatible, Multiplexing
Libraries: Targeted Sequencing Library
Multiplex Capability: 12- to 3,072-plex per well
Product Line: Ion AmpliSeq™
Sample Type: DNA (Genomic), DNA from FFPE Tissues, Tissue (FFPE)
Sequencing Type: Genome & DNA Sequencing
Species: Human
Starting Material: DNA
Starting Material Amount: 10 ng gDNA
Technique: Amplicon Sequencing
Workflow Step: Library Generation
For Use With (Equipment): Ion GeneStudio S5 System, Ion GeneStudio™ S5 System, Ion PGM™ System, Ion Proton™ System, Ion S5™ XL System, Ion OneTouch™ 2 System, Ion Chef™ System

Contents & storage

7 tubes. Store at -30 to -10 °C
1 tube Low TE Buffer. Store at room temperature