VeriFiler™ Direct/Identifiler™ Direct Paternity Testing Bundle

Catalog number: 4480504

Applied Biosystems™  Related applications: Forensics

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The VeriFiler™ Direct/Identifiler™ Direct Paternity Testing Bundle contains these three products in a discounted combo pack:

VeriFiler™ Direct PCR Amplification Kit
AmpFℓSTR® Identifiler™ Direct PCR Amplification Kit
Prep-N-Go™ Lysis Buffer (Buccal Swabs)

The VeriFiler™ Direct and Identifiler™ Direct kits are PCR amplification kits that allow for direct amplification from the buccal swabs and FTA®-treated paper cards commonly used in paternity testing applications (buccal samples require the use of the included Prep-N-Go™ Lysis Buffer). Direct amplification eliminates the need for sample extraction and quantification, which can greatly increase your lab efficiency and decrease the required labor and technician hands-on time.

When used together, the VeriFiler™ Direct and Identifiler™ Direct PCR amplification kits allow for the amplification of 21 highly discriminatory STRs plus the sex determining marker amelogenin in two separate PCR amplifications. In addition, the two kits contain 4 overlapping loci (3 STRs and amelogenin) as a quality control measure to help ensure cross-amplification genotyping consistency between the two reactions.

These two kits together offer you:

High discrimination—targets these 21 STR loci plus amelogenin in two PCR reactions: CSF1PO, D5S818, D7S820, D13S317, TPOX, D3S1358, D8S1179, D16S539, D18S51, D21S11, FGA, TH01, vWA, D2S1338, D19S433, D6S1043, D1S1656, D2S441, D10S1248, D12S391, D22S1045
High efficiency—employs a direct amplification workflow from these common sample types used in paternity testing: buccal swabs, FTA card punches, gDNA extracts
High compatibility—works with your existing Applied Biosystems® CE instrumentation and data analysis software packages: Veriti® and 9700 thermal cyclers, 3130 and 3500 genetic analyzers, GeneMapper® ID and GeneMapper® ID-X software

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.
For Paternity Use Only. Not for use in Research or Clinical Diagnostic applications.


Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, VIC®, FAM™, PET™, LIZ™
Product Size: 200 tests
Amplicon Size: <300 bp
Sample Volume: 25 µl⁄reaction
Reaction Format: SuperMix or Master Mix
For Use With (Equipment): GeneAmp™ 9700, Veriti® Thermal Cycler, 3500xL Genetic Analyzer, 3500 Genetic Analyzer, 3130 Genetic Analyzer, 3130xl Genetic Analyzer
Matrix and Size Standards: Size Standard: GeneScan&trade 600 LIZ® Size Standard v2.0, Matrix Standard: DS-33 Matrix Standard Kit (Dye Set G5)

Contents & storage

200 tests VeriFiler™ Direct
200 tests Identifiler™ Direct
200 tests Prep-N-Go™ lysis buffer


Manuals & protocols