Applied Biosystems™

POROS™ GoPure™ HQ Pre-packed Column, 0.5 x 5 cm, 1 mL

Catalog number: 4481315
Applied Biosystems™

POROS™ GoPure™ HQ Pre-packed Column, 0.5 x 5 cm, 1 mL

Catalog number: 4481315
Catalog Number
Unit Size
1 column
Price (USD)
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44813151 column
Product Overview
Citations & References
Additional Information

GoPure™ 0.5 cm pre-packed columns are intended for the screening of POROS™ ion exchange resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

  • Dimensions of 0.5 cm x 5 cm, with a nominal bed volume of 1 mL, providing a uniform screening platform
  • Direct connection to standard chromatography systems
  • Packed with POROS™ high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The resin in this GoPure™ HQ 50 μm Column has a quaternized polyethyleneimine functional group for strong anion exchange chromatography.

  • Convenient—ready-to-run columns simplify and speed POROS™ resin evaluations
  • Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
  • Simplified scalability—the consistency and range of the GoPure™ family of pre-packed columns simplifies process scale-up and process modeling

GoPure™ 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS™ resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended ≥3 min residence time (0.3 mL/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the GoPure 0.5 cm Operating Instructions below.

Other POROS™ anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS™ High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure™ Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 mL to 6.3 liters.

For Research Use Only. Not for use in diagnostic procedures.


Diameter (Metric)
0.5 cm
For Use With
Length (Metric)
5 cm
Max. Pressure
30 bar
Particle Size
50 μm
Stationary Phase
Quaternized Polyethyleneimine
Product Line
High-throughput Compatibility
Automated Protocols
Sample Type
Biopharmaceutical Purification Sample, Cell Culture Supernatants, Serum
Volume (Metric)
1 mL
Column Type
Anion Exchange

Contents & Storage

Shipped in 20% ethanol at 2°C to 30°C.


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