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Ion Torrent™
Ion Library Equalizer™ Kit
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and libraryWeitere Informationen
| Katalognummer | Menge |
|---|---|
| 4482298 | 96 Reaktionen |
Katalognummer 4482298
Preis (EUR)
942,00
Each
Menge:
96 Reaktionen
Preis (EUR)
942,00
Each
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.
The kit workflow consists of three simple steps:
1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer
Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability
Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency
Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization
Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.
Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit
Optional:
Ion Xpress™ Barcode Adapters Kits
The kit workflow consists of three simple steps:
1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer
Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability
Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency
Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization
Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.
Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit
Optional:
Ion Xpress™ Barcode Adapters Kits
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Zur Verwendung mit (Anwendung)Amplicon Sequencing
Zur Verwendung mit (Geräte)Ion PGM™ System, Ion Proton™ System, Ion Personal Genome Machine (PGM™) System
Format96-Well-Platte
BibliothekenFragment Library, Targeted Sequencing Library, Normalized Library
Multiplex-FunktionNo Limit on Multiplexing
ProdukttypLibrary Equalizer Kit
Menge96 Reaktionen
ProbentypJeder Probentyp
ProbenvolumenFragment or Targeted AmpliSeq Libraries
SequenzierungstypEpigenetic Sequencing, Genome & DNA Sequencing
SpeziesAlle Arten
DurchsatzUp to 10 Samples
ArbeitsablaufschrittLibrary Barcoding, Library Generation, Template Preparation
ProduktlinieEqualizer
AusgangsmaterialDNA
Unit SizeEach
Inhalt und Lagerung
• Equalizer Primers - store at 2–8°C
• Equalizer Capture - store at 2–8°C
• Equalizer Wash Buffer - store at room temperature (can be stored at 2–8°C)
• Equalizer Elution Buffer - store at 2–8°C
• Equalizer Beads - store at 2–8°C
Note kits are shipped at ambient temperature store each component at specified temperatures.
• Equalizer Capture - store at 2–8°C
• Equalizer Wash Buffer - store at room temperature (can be stored at 2–8°C)
• Equalizer Elution Buffer - store at 2–8°C
• Equalizer Beads - store at 2–8°C
Note kits are shipped at ambient temperature store each component at specified temperatures.