Ion Library Equalizer™ Kit

Catalog number: 4482298

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.

The kit workflow consists of three simple steps:

1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer

Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer®-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability

Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer®-based workflow (up to 10 samples)
• Reduced AMPure® XP reagent usage compared to Agilent 2100 Bioanalyzer®-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency

• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization

The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.

Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (
Ion Plus Fragment Library Kit
Ion Xpress™ Plus Fragment Library Kit

Ion Xpress™ Barcode Adapters Kits
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System, Ion Proton™ System
Library Type: Fragment Library, Targeted Sequencing Library, Normalized Library
Workflow Step: Library Generation, Library Barcoding, Template Preparation
Starting Material: DNA
Sequencing Type: Epigenetic Sequencing, Genome & DNA Sequencing
Species: All Species
Technique: Amplicon Sequencing
Product Size: 96 reactions
Multiplex Capability: No Limit on Multiplexing
Sample Type (Specific): Any Sample Type
Starting Material (Amount): Fragment or Targeted AmpliSeq Libraries

Contents & storage

1 tube Equalizer Primers - Store at 2-8 °C
1 tube of Equalizer Capture - Store at 2-8 °C
1 tube of Equalizer Wash Buffer - Store at 2-8 °C
1 tube of Equalizer Elution Buffer - Store at 2-8 °C
1 tube of Equalizer Beads - Store at 2-8 °C

Note kits are shipped at ambient temperature store each component at specified temperatures.


Manuals & protocols

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