The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.
The kit workflow consists of three simple steps:
1) Library amplification with Equalizer™ Primers 2) Library capture onto Equalizer™ Beads 3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer
Simple Workflow • Seamlessly integrates into your existing library preparation workflow • Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow • Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries • Minimizes library-to-library variability
Cost Effective • No additional capital equipment required • Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples) • Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow • Long shelf life: up to 18 months • Convenient 96-reaction kit helps reduce ordering frequency
Flexible • Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors • Use with a single sample or in a 96-well plate format • Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization
Includes: The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.
Required for Workflow: Ion AmpliSeq™ Library Kit 2.0 Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com) --OR-- Ion Plus Fragment Library Kit --OR-- Ion Xpress™ Plus Fragment Library Kit
Optional: Ion Xpress™ Barcode Adapters Kits
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Application)
For Use With (Equipment)
Ion PGM™ System, Ion Proton™ System, Ion Personal Genome Machine (PGM™) System
Fragment Library, Targeted Sequencing Library, Normalized Library
• Equalizer Primers - store at 2–8°C • Equalizer Capture - store at 2–8°C • Equalizer Wash Buffer - store at room temperature (can be stored at 2–8°C) • Equalizer Elution Buffer - store at 2–8°C • Equalizer Beads - store at 2–8°C
Note kits are shipped at ambient temperature store each component at specified temperatures.