Ion Torrent™

Ion Library Equalizer™ Kit

Catalog number: 4482298
Ion Torrent™

Ion Library Equalizer™ Kit

Catalog number: 4482298
Catalog Number
4482298
Unit Size
96 reactions
Price (USD)
Price: 764.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
448229896 reactions
Price: 764.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.

The kit workflow consists of three simple steps:

1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer

Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer™-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability

Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer™-based workflow (up to 10 samples)
• Reduced AMPure™ XP reagent usage compared to Agilent 2100 Bioanalyzer™-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency

Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization

Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.

Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit

Optional:
Ion Xpress™ Barcode Adapters Kits
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line
Equalizer™
Starting Material
DNA
Format
96-well Plate
For Use With (Application)
Amplicon Sequencing
For Use With (Equipment)
Ion PGM™ System, Ion Proton™ System, Ion Personal Genome Machine (PGM™) System
Libraries
Fragment Library, Targeted Sequencing Library, Normalized Library
Multiplex Capability
No Limit on Multiplexing
Product Type
Library Equalizer Kit
Sample Type
Any Sample Type
Sample Volume
Fragment or Targeted AmpliSeq Libraries
Sequencing Type
Epigenetic Sequencing, Genome & DNA Sequencing
Species
All Species
Throughput
Up to 10 Samples
Workflow Step
Library Barcoding, Library Generation, Template Preparation

Contents & Storage

• Equalizer Primers - store at 2–8°C
• Equalizer Capture - store at 2–8°C
• Equalizer Wash Buffer - store at room temperature (can be stored at 2–8°C)
• Equalizer Elution Buffer - store at 2–8°C
• Equalizer Beads - store at 2–8°C

Note kits are shipped at ambient temperature store each component at specified temperatures.

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