QuantStudio™ 3D Digital Chip Kit - FAQs

This will depend on the concentration range of the sample used. The QuantStudio 3D Digital PCR System has application- and concentration-specific requirements for technical replicates. At a low concentration, more replicates will be needed to have confidence in the data. Also, due to the nature of the application, replicates would be needed when studying rare mutations.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

The current QuantStudio 3D Digital PCR Chip allows for one sample per chip; however, duplexing allows analysis of two targets per chip.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

The QuantStudio 3D Digital PCR System can read the digital chip in less than 1 minute. In fact, if you are running multiple chips, you can run the next chip in the series when the instrument touch screen displays the Analyzing Chip screen.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

After thermal cycling, the QuantStudio 3D Digital PCR 20K Chips can remain on the sample block for up to 24 hours. Read the QuantStudio 3D Digital PCR 20K Chips within 1 hour after you remove them from the GeneAmp 9700 PCR System sample block.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Using the Chip Carrier on the GeneAmp 9700 Dual Flat Block, up to 24 QuantStudio 3D Digital Chips can be run at one time. Please refer to Chapter 3 in the QuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) for more details on loading the chips for thermal cycling.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

The QuantStudio 3D Digital PCR 20K Chip is a 10 mm2 high-density reaction plate that consists of a single array of 20,000 reaction wells, where each well can accommodate an 865 pL reaction. Hydrophobic coatings on the chip surface enable loading and isolation of independent PCR reactions within the reaction wells. Please see an image of the chip in the QuantStudio 3D Digital PCR System User Guide, found here: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0007720.pdf

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Yes, the current Lids will work on the new v2 Chips.