ProFlex™ 3 x 32-well PCR System

Catalog number: 4484073

Applied Biosystems™  Related applications: PCR | Reverse Transcription

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The ProFlex™ 3x32-Well PCR System is the latest in our line of Applied Biosystems® thermal cyclers. The ProFlex™ system combines the reliability and performance you’ve come to expect from Applied Biosystems® instruments with the flexible configuration and control features that adapt to your research needs.

Request a demo.

The ProFlex™ 3x32-Well PCR System allows you to:

• Run three experiments at once with the 3x32-well, 0.2-mL block
• Interchange the 3x32-well block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex™ blocks

Independent Control
Share your instrument with 2 other people or run 3 experiments at once with 3 independently controlled 32-well modules.

Extended Warranty Packages
Protect your instrument and your investment with the ProFlex PCR System Extended Warranty Packages. Save up to 25% off the individual instrument and extended warranty list prices when purchased together. Learn more about the packages >

Interchangeable Blocks
If your research requirements change in the future, or if you share the system with someone who requires a different format, simply remove the 3x32-well block from the top of the instrument and exchange it with another block type. This step takes typically less than 15 seconds, and requires no tools or kits.

Mobile App Connectivity
Have you ever set up all of your PCR reactions, only to find out that the thermal cycler is already in use? Check its availability with a mobile app before you get to the lab. Also download experimental report files, receive alerts, and more! Download our PCR Essentials app updated with this functionality.

Simulation Modes
The ProFlex™ PCR system is equipped with Simulation Modes that mimic your old thermal cycler's ramp rate. Simulation modes are available for MJ Research PTC 200, Bio-Rad® C1000, Bio-Rad® MyCycler™, Eppendorf® Mastercycler®, Applied Biosystems® 9700, Applied Biosystems® 9600, and Applied Biosystems® Veriti® systems.

VeriFlex™ Blocks: Enhanced PCR Functionality
VeriFlex™ blocks are controlled by multiple Peltier elements (2 separate Peltier elements per 32-well module). This gives you the ultimate control of your block for precise, "better-than-gradient" optimization experiments, and for running multiple reaction conditions at once for precise incubations and other non-PCR workflows.

8.4" Touch Screen Control
The simple and intuitive touchscreen interface allows you to program with ease, and limits the amount of time spent training yourself and others how to use the system.
For Research Use Only. Not for use in diagnostic procedures.


Format: 0.2 ml tubes, 12-strip wells
Weight: 18.75 kg (41 lb)
Capacity: 3 x 32-well x 0.2 ml tubes
Dimensions: 33 cm (13 in) (W) x 27 cm (11 in) (H) x 57 cm (22 in) (D)
Block Format: Interchangeable
Product Size: 1 instrument
Reaction Speed: Standard, Fast
Program Features: Program overwrite protection, Auto re-start (after power outages)
Sample Ramp Rate: ± 4.4 °C⁄sec
Display Interface: Touchscreen (8.4 in. TFT LCD)
Instrument Memory: USB and On-board
Power Requirements: 100-240V, 50-60 Hz Max: 950 VA
Peak Block Ramp Rate: 6.0°C⁄sec
Temperature Accuracy: ±0.25°C (35°C to 99.9°C)
Reaction Volume Range: 10-80 µl
Temperature Uniformity: <0.5 °C (20 sec after reaching 95 °C)
For Use With (Equipment): ProFlex™ PCR System
Temperature Range (Metric): 0 to 100.0 °C
High Throughput Compatibility: High Throughput-Compatible


Manuals & protocols

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