Ion Chef™ Instrument

Catalog number: 4484177

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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The Ion Chef System is the next generation of workflow simplification products for the Ion S5 System, Ion Proton System, and Ion PGM System. It features automated Ion AmpliSeq library preparation in addition to automated, high-throughput template preparation and reproducible chip loading for all libraries. With typically less than 30 minutes of total hands-on time, an intuitive touch-screen interface, and significantly higher productivity than the Ion OneTouch 2 System, the Ion Chef System makes the Ion Torrent sequencing workflow accessible for every lab.

The Ion Chef System:
• Simplifies the workflow
• Minimizes sources of user-introduced variability
• Helps save time and labor
• Supports sequencing preparation for the Ion S5, Ion PGM, and Ion Proton systems and chips

The Ion Chef System features:
• Automated Ion AmpliSeq library preparation, template preparation, and chip loading
• Cartridge-based reagent system
• Sample- and reagent-tracking capability
• Intuitive user interface
• On-board diagnostics

Simplify the Ion Torrent NGS workflow
The Ion Chef System simplifies the Ion Torrent next-generation sequencing workflow by integrating several manual and instrument steps into a single process. The Ion Chef System workflow incorporates: AmpliSeq library preparation, amplification mix preparation, emulsification, amplification, breaking, enrichment, washing, sequencing prep, and preparation & loading of up to two chips. The automated sample preparation provides easy access for users with any experience level.

Minimize variability & load chips reproducibly
The Ion Chef System provides reproducible chip loading by automating multiple manual chip-loading steps. The sophisticated visual system inside the system is designed to help reduce user-introduced error and variability by providing barcode reading and positive ID of samples, chips, and reagents. The visual system performs the set-up check before the run, as well as the reagents and consumables load and unload check, calibration, and diagnostics.

Save time and labor
The Ion Chef System enables significant time and labor savings by offering quick and easy set-up that typically requires less than 30 minutes hands-on time and loading of up to two chips in a single-day workflow.

Support for all Ion Torrent sequencing workflows
The flexible Ion Chef System is designed to provide automated and consistent AmpliSeq library preparation, template preparation for sequencing on Ion S5, Ion PGM, and Ion Proton systems using any of the corresponding chips, or template preparation and sequencing chemistries.

For Research Use Only. Not for use in diagnostic procedures.


Chip Compatibility: Ion 314™ Chip (200 bases Q1 2014, 400 bases 2014)
Ion 316™ Chip (200 bases Q1 2014, 400 bases 2014)
Ion 318™ Chip (200 bases Q1 2014, 400 bases 2014)
Ion PI™ Chip (200 bases Q1 2014)
Ion PII™ Chip* (TBA)
Current Limit: 14 A (max)
Dimensions: 28 x 22 x 28 in (71 x 55 x 71 cm)
Frequency: 50⁄60 Hz
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible
Operating Environment: Temperature: 15°C-25°C
Operating System: Ion Personal Genome Machine (PGM™) System, Ion Proton™ System
Power Limit: 1,350 W
Product Line: Ion Chef™
Product Size: 1 system
Run Time: Varies with chip type and read length selected
Voltage Limit: 100 V (min) to 240 V (max)


Manuals & protocols