Ion Chef™ Instrument

Description

The Ion Chef System is the next generation of workflow simplification products for the Ion S5, Ion Proton, and Ion PGM systems. The Ion Chef System provides automated library preparation, template preparation, and chip loading for users at any experience level. In less than 15 minutes of up-front hands-on time and with the use of pre-packaged library preparation reagent kits, the Ion Chef System provides a convenient walk-away workflow resulting in equalized, pooled libraries ready for templating. The automated workflow supports Ion AmpliSeq one- and two-pool designs. See www.AmpliSeq.com to design your own custom panels. Compared to the four hours of hands-on time required with the Ion OneTouch 2 System, the Ion Chef System enables significantly higher productivity. Again, in less than 15 minutes of hands-on time, the fully automated template preparation provides two chips ready for sequencing.

The Ion Chef System:
• Simplifies the workflow
• Minimizes sources of user-introduced variability
• Helps to save time and labor
• Supports sequencing preparation for the Ion S5, Ion PGM, Ion Proton systems

The Ion Chef System features:
• Automated Ion Ampliseq library preparation, template preparation, and chip loading
• Cartridge-based reagent system
• Sample and reagent-tracking capability
• Intuitive user interface
• On-board diagnostics

Simplify the Ion Torrent NGS workflow
The Ion Chef System simplifies the Ion Torrent next-generation sequencing (NGS) workflow by integrating several manual and instrument steps into a single process. The Ion Chef System workflow now incorporates: all steps of library preparation: targeted PCR amplification with respective primer panel, partial digestion of primer sequences, adapter/barcode ligation, purification, library equalization and pooling, as well as all steps of template preparation and chip loading. The simple, automated workflow provides easy access for users of any experience level.

Minimize variability and load chips reproducibly
The Ion Chef System provides reproducible chip loading by automating multiple manual chip-loading steps. The sophisticated visual system inside the Ion Chef System is designed to help reduce user-introduced error and variability by providing barcode reading and positive identification of samples, chips, and reagents. The visual system performs the set-up check before the run, as well as the reagents and consumables load and unload check, calibration, and diagnostics.

Save time and labor
The Ion Chef System enables significant time and labor savings by offering quick and easy set-up for both library preparation and templating that typically requires less than 15 minutes of hands-on time for each step.

Support for all Ion Torrent sequencing workflows
The flexible Ion Chef System is designed to provide automated and consistent Ion AmpliSeq library preparation and template preparation for sequencing on Ion S5, Ion Proton, and Ion PGM systems using any of the corresponding chips or template preparation and sequencing chemistries.

Services and support
Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

Spécifications

Chip Compatibility:	Ion PI™ Chip v3, Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, Ion 318™ Chip v2 BC, Ion 520™ Chip, Ion 530™ Chip, Ion 540™ Chip
Current Limit:	14 A (max)
Dimensions:	28 x 22 x 28 in (71 x 55 x 71 cm)
For Use With (Equipment):	Ion PGM™ System, Ion Proton™ System, Ion S5™ System, Ion S5™ XL System
Frequency:	50⁄60 Hz
High Throughput Compatibility:	Automated Protocols, High Throughput-Compatible
Operating Environment:	Temperature: 15°C-25°C
Operating System:	Ion Personal Genome Machine (PGM™) System, Ion Proton™ System
Power Limit:	1,350 W
Product Line:	Ion Chef™
Product Size:	1 system
Run Time:	Varies with chip type and read length selected
Voltage Limit:	100 V (min) to 240 V (max)