This product is now part of a food safety range that includes Thermo Scientific™ culture media, molecular solutions, and other products for your complete microbiological workflow. In US, Canada and Europe, order this product from Thermo Fisher Scientific microbiology. >> Order now
This product has a minimum order quantity of 10 and a lead time associated. See alternative product(s).

Custom TaqMan™ STEC O26 & O111 Assay, MLG

Catalog number:  4485008

 Related applications: Food & Beverage

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


This Taqman® assay provides a simple, reliable, and rapid procedure for detecting the presence of a specific bacterial or viral target. PCR is used to amplify a DNA target unique to the microorganism, then TaqMan® probe is used to detect and quantify the target in a easy-to-use, single tube format. This assay enables microbial detection capabilities with high-confidence results to will help accelerate your food and environmental testing needs. The assay design is based on "Detection and Isolation of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) from Meat Products", method #5B.03 from the Food Safety and Inspection Service (FSIS) Molecular Laboratory Guidebook (MLG). The assay has been optimized for use with the included Environmental Master Mix; a negative control is not included with this assay.

Features of TaqMan® assays:

• Include an internal positive control to monitor your PCR
• Suitable for single or multiplexed assays
• Easy-to-use, single tube format
• Run using universal conditions- analyze multiple assays in the same run
• Robust assay design provides highly accurate, reproducible, and reliable results
• Include passive internal reference based on proprietary ROX™ dye for precise data analysis

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

Custom assays have been bioinformatically developed and tested for performance. Validation services for specific samples types are provided by our Custom Solutions team. Please contact your local Thermo Fisher Scientific representative for more information.
For Testing of Food and Environmental Samples Only.


Comments: Includes Master Mix
Form: Liquid
Recommended Applications: Polymerase Chain Reaction
Target Organism Class: E. coli
Detection Method: Primer-Probe Detection
Reaction Speed: Standard
Product Size: 100 reactions
Species: All Species

Contents & storage

• 10x Assay Mix (Store at -5°C to -30°C.)
• 2x Environmental Master Mix (Store at -5°C to -30°C upon receipt. Store at 2–8°C after use.)

These reagents are sufficient for 100 reactions.


Manuals & protocols

Recommended products