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TaqMan® Verotoxin-producing Escherichia coli VT1 Assay, HPA

Catalog number: 4485016

 Related applications: Food & Beverage

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This Taqman® assay provides a simple, reliable, and rapid procedure for detecting the presence of a specific bacterial or viral target. PCR is used to amplify a DNA target unique to the microorganism, then TaqMan® probe is used to detect and quantify the target in a easy-to-use, single tube format. This assay enables microbial detection capabilities with high-confidence results to will help accelerate your food and environmental testing needs. The assay design is based on a publication by Nielsen, et al. [Nielsen EM, Andersen MT (2003) J Clin Micro 41:2884-2983] and validated by the Health Protection Assocation (HPA). The assay has been optimized for use with the included Environmental Master Mix; a negative control is not included with this assay.

Features of TaqMan® assays:

• Include an internal positive control to monitor your PCR
• Suitable for single or multiplexed assays
• Easy-to-use, single tube format
• Run using universal conditions- analyze multiple assays in the same run
• Robust assay design provides highly accurate, reproducible, and reliable results
• Include passive internal reference based on proprietary ROX™ dye for precise data analysis

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.
For Testing of Food and Environmental Samples Only.


Form: Liquid
Comments: Includes Master Mix
Target Organism Class: E. coli
Validated Application: Polymerase Chain Reaction
Detection Method: Primer-Probe Detection
Reaction Speed: Standard
Species: All Species
Product Size: 100 reactions

Contents & storage

• 10x Assay Mix (Store at -5°C to -30°C.)
• 2x Environmental Master Mix (Store at -5°C to -30°C upon receipt. Store at 2–8°C after use.)

These reagents are sufficient for 100 reactions.


Manuals & protocols