Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS™ CaptureSelect™ GCSF HPLC columns incorporate the advantages of high-speed POROS™ chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three-minute quantitation of granulocyte colony-stimulating factor (GCSF).
• Broad utility—POROS™ A, G, and CaptureSelect™ affinity ligands address most biological drug classes • Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins, fusion proteins • Hands-off operation—HPLC method can be automated for streamlined operation • Robust—allows for high-performance purification over hundreds of chromatography cycles
High Speed with High Specificity The combination of CaptureSelect™ GCSF affinity ligand and POROS™ 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid titer determination and small-scale purification of GCSF.
POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and allows for an eluted product that is highly purified and suitable for further analysis, including mass spectrometry.
Automation POROS™ affinity chromatography columns are suitable for use with HPLC systems, which allows for the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.
Multiple Cycles Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime can result in strong re-use performance. POROS™ affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Capacity (Metric)
0.8 mL
Matrix
POROS
Elution Volume Max
29 column volumes
Elution Volume Min
14 column volumes
For Use With (Equipment)
FPLC
High-throughput Compatibility
Automated Protocols
Max. Operating Pressure
2600 psi, 180 bar
Particle Size
20 μm
Product Line
CaptureSelect™, POROS™
Quantity
1 Column
Target
Protein
Type
Column
Stationary Phase
CaptureSelect™ GCSF
Format
Pre-packed Column
Column Type
Affinity
Contents & Storage
Column includes EZ grip fittings and column test certificate. Store at 2–8°C. DO NOT FREEZE.