POROS® CaptureSelect® IgA Affinity Column, 4.6 x 100 mm

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Description

Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS® CaptureSelect™ IgA HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of CaptureSelect™ ligands. The combination allows three-minute quantitation of IgA.

Broad utility—POROS® A, G, and CaptureSelect™ affinity ligands address most biological drug classes
Rapid—POROS® resins offer fast quantitation or purification of immunoglobulins, fusion proteins
Hands-off operation—HPLC method can be automated for streamlined operation
Robust—allows for high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ IgA affinity ligands and POROS® 20 µm resin provides a high flow rate, high performance chromatography solution for the rapid titer determinations and small-scale purification of IgA.

POROS® perfusion chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and allows for an eluted product that is highly purified and suitable for further analysis, including mass spectrometry.

Automation
POROS® affinity chromatography columns are suitable for use with HPLC systems, which allows for the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime can result in strong re-use performance. POROS® affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Affinity Column
Elution Volume - max: 29 column volumes
Elution Volume - min: 14 column volumes
For Use With (Equipment): FPLC
Format: Prepacked Column
High Throughput Compatibility: Automated Protocols
Immobilized Phase: CaptureSelect™ IgA
Maximum Operating Pressure: 2600 PSI, 180 Bar
Particle Size: 20 micron
Product Size: 1 column
Support Matrix: POROS
Target Molecule: Protein

Contents & storage

Column includes EZ grip fittings and column test certificate.
Store at 2–8°C. DO NOT FREEZE.

Documents

Manuals & protocols

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