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TaqMan® GAPDH Assay, JUN® dye/QSY® probe This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 4485712

Applied Biosystems™  Related applications: Real Time PCR (qPCR)

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The Applied Biosystems® TaqMan® GAPDH Assay with JUN® dye-labeled QSY® probe provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. The assay consists of a JUN® dye-labeled QSY® probe plus sequence-specific forward and reverse primers, and it can be used for multiplex or singleplex PCR reactions.

Benefits of this assay include:

• Eliminate assay design time by using pre-designed primers and pre-designed JUN® dye-labeled TaqMan® probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC® dye-labeled predesigned gene expression assays

Save time in assay design, formulation, and testing by using the TaqMan® GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

The JUN® dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN® dye) due to an overlap of spectra. TaqMan® Multiplex Master Mix with MUSTANG PURPLE® dye is recommended.
For Research Use Only. Not for use in diagnostic procedures.


Format: Tube(s)
Species: Human
Includes: Primer-Probe Set(s)
Gene Symbol: GAPDH
Target Gene: Glyceraldehyde-3-phosphate dehydrogenase
Label or Dye: JUN™
Product Size: 250 reactions
Amplicon Size: 118
Accession Number: NM_002046.3
Internal Probe Modification: QSY® Non-Fluorescent Quencher (3')
Green Features: Less waste, Sustainable packaging

Contents & storage

Store in freezer (-5 to -30°C).


Manuals & protocols

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