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Applied Biosystems™

TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited

Green features
Catalog number: 4485713
Applied Biosystems™

TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited

Green features
Catalog number: 4485713
Catalog Number
4485713
Unit Size
250 reactions
Price (USD)
325.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4485713250 reactions
325.00
Your Price:
***
Product Overview
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The Applied Biosystems™ TaqMan™ GAPDH Assay with JUN™ dye-labeled QSY™ probe (primer limited) provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of a JUN™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers. The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress GAPDH.

Benefits of this assay include:

• Eliminate assay design time by using pre-designed primers and pre-designed JUN™ dye-labeled TaqMan™ probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays
• Formulated at a primer-limited concentration for use in multiplex reactions

Save time in assay design, formulation, and testing by using the TaqMan™ GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high GAPDH expressors.

The JUN™ dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN™ dye) due to an overlap of spectra. TaqMan™ Multiplex Master Mix with MUSTANG PURPLE™ dye is recommended.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NM_002046.3
Amplicon Size
118
Detection Method
Primer-Probe
Format
Tube(s)
GC-Rich PCR Performance
High
No. of Reactions
250 Reactions
PCR Method
qPCR, RT-PCR
For Use With (Application)
Real Time PCR (qPCR)
Gene Symbol
GAPDH
Green Features
Sustainable packaging
Includes
Primer-Probe Set(s)
Internal Probe Modification
QSY™ Non-Fluorescent Quencher (3')
Label or Dye
JUN™
No. of Tests
250 Reactions
Product Line
TaqMan™
Product Type
Gapdh Assay
Species
Human
Sufficient For
250 Reactions
Target Gene
Glyceraldehyde-3-phosphate dehydrogenase

Contents & Storage

Store in freezer (-5 to -30°C).

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