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View additional product information for QuantStudio™ 3D Digital PCR Master Mix - FAQs (4485718)
61 product FAQs found
解析ソフトのAnalysisSuiteに稀釈倍率を入力していれば、最終的に稀釈倍率を考慮した結果（copies/μL）が算出されます。稀釈倍率については、2種類の稀釈について考慮する必要があります。第一は反応液における稀釈で、第2はdigital PCR反応液に加える前の稀釈です。例えば、サンプル原液の10倍希釈液を、トータル16ulの反応液に1ul加えた場合、ソフトウェアに入力するべき稀釈倍率は1:10 (0.1)と1:16 (0.0625)なので、0.0625 x 0.1 = 0.00625となります。この数値をAnalysisSuite softwareの“Dilution”カラムに入力すれば、サンプル原液の結果（copies/μL）が算出されます。
AnalysisSuite softwareはGoogle Chrome v4.0以降に最適化しております。Firefoxでも動作いたしますが、Internet Explorerは推奨していません。
ファームウェアのアップデート手順詳細はQuantStudio 3D Digital PCR System User Guideの"Chapter A: Maintenance"をご参照ください。
これはご使用になるサンプル濃度のレンジに依存します。QuantStudio 3D Digital PCR Systemではアプリケーションやサンプル濃度に応じた要件がございます。低濃度サンプルの場合、正確なデータを出すにはより多くの反復が必要になります。また、レアな体細胞変異を検出する場合にも反復は必要になります。
現在のQuantStudio 3D Digital PCR Chipは1チップあたり1サンプルでの使用となりますが、duplex反応にすることで1チップあたり2ターゲットの解析は可能です。
ネットワーク接続の詳細手順はQuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) のAppendix Bに記載されていますので、ご参照ください。
QuantStudio 3D Digital PCR Systemは1分以下でチップをリードいたします。もし複数枚のチップをランニングしている場合、本体のスクリーンに表示が出れば次のチップのリードを行うことが可能です。
サーマルサイクリング後、QuantStudio 3D Digital PCR 20K Chipsはサンプルブロック上で24時間まで保存することが可能です。 GeneAmp® 9700 PCR Systemのサンプルブロックから移したら1時間以内にリードしてください。
GeneAmp® 9700 Dual Flat Blockにチップキャリアーを使用して、24枚のQuantStudio 3D Digital PCR Chipsを同時にランニングすることが可能です。詳細についてはQuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf)をご確認ください。
QuantStudio 3D Digital PCR 20K ChipsやChip Case Lidsを扱う際にはグローブをしてください。手からの油脂が増幅やイメージングの妨げとなることがあります。詳細の手順は、QuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf)のChapter 2 loading the digital chipsをご参照ください。
ファームウェアバージョンは3.12以降である必要があります。GeneAmp® 9700 PCR Systemのファームウェアバージョンは最初に電源を入れた際のメインディスプレイに表示されます。アップグレードの必要がある場合にはダウンロードしてください。
QuantStudio 3D Digital Master Mixのみが使用可能です。
QuantStudio 3D instrumentはチップの読み取りと一次解析のみを行います。サーマルサイクリングはデュアルフラットブロックのProflex もしくは GeneAmp® 9700 PCRで行う必要があります。
QuantStudio 3D Digital PCR ChipsはデュアルフラットブロックのProflex もしくは GeneAmp® 9700 PCRでご使用いただけます。
UV硬化樹脂は、正しく保管いただければ開封から3カ月間使用可能です。遮光バックに入れて暗所にて保存ください。
Tilt BaseはPCR装置を11°傾ける為のものです。この角度がQuantStudio 3D Digital PCR 20K Chipsの最適なパフォーマンスに必要となります。
一度開封したImmersion fluidは60分以内に使用してください。後で使用するためにシリンジを分解することはできません。
QuantStudio 3D Digital PCRシステムはFAM, VIC®, そして ROX に最適化されたフィルターを有しています。
QuantStudio 3D Digital PCR 20K Chipは10平方ミリの高密度反応チップ20,000反応ウェルが配置され、各ウェルの反応容量は865pLです。チップ表面は疎水性コーティングがされており、反応液ローディング後の各ウェルが独立して存在することを可能としています。詳細につきましては QuantStudio 3D Digital PCR System User Guide（http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf）をご参照ください。
設置条件詳細に関しましてははQuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) のAppendix Bに記載されていますので、ご参照ください。
デジタルPCRは、レアな体細胞変異の検出、発現量の絶対定量、ウイルス量、次世代シーケンサーのライブラリー定量等に使用できます。
QuantStudio 12K Flex systemの OpenArray® block と、QuantStudio 3D system でデジタル PCRが可能であります。
StepOne, StepOnePlus, ViiA 7, QuantStudio 3/5/6/712K Flex システムは、制御コンピューターなしでも、装置のタッチスクリーンから直接ランが可能です。
QuantStudio 12K Flex システムのOpenArray®ブロック、QuantStudio 3D システムがデジタルPCRが可能な装置です。
レアアレルの検出、遺伝子発現の絶対定量、ウイルスの定量、および次世代シーケンサーにおけるライブラリの定量に用いることができます。
If you include all of the necessary dilution factors into the AnalysisSuite software, the software will give you the copies/µL in your stock. There are 2 dilutions you need to take into account. The first is the dilution of the sample in the reaction. The second is the dilution of the stock that you make before adding it to the digital PCR reaction.
For example, say you want to add 1 µL of a sample that has been diluted 1:10 from the stock. If you add 1 µL of your sample to a 16 µL (final volume) reaction, the dilution factor of the sample is 1:16 or 1/16 = 0.0625. Since the stock has also been diluted 1:10 (0.1), you also need to factor this in. The final dilution factor to enter into the software is 0.0625 x 0.1 = 0.00625 (1:160). As you can see below, you can use either annotation to indicate the dilution factor in the AnalysisSuite software. If you enter that value into the “Dilution” column, the software will give you the copies/µL in your starting material (stock).
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
AnalysisSuite software is optimized for Google Chrome v4.0 or later. Firefox also works, but Internet Explorer is not recommended.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Please follow the procedures starting in "Chapter A: Maintenance" of the QuantStudio 3D Digital PCR System User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) to update the instrument firmware.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
This will depend on the concentration range of the sample used. The QuantStudio 3D Digital PCR System has application- and concentration-specific requirements for technical replicates. At a low concentration, more replicates will be needed to have confidence in the data. Also, due to the nature of the application, replicates would be needed when studying rare mutations.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The current QuantStudio 3D Digital PCR Chip allows for one sample per chip; however, duplexing allows analysis of two targets per chip.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Please follow the procedures described starting in Appendix B of the QuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) to connect the instrument to a network.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The QuantStudio 3D Digital PCR System can read the digital chip in less than 1 minute. In fact, if you are running multiple chips, you can run the next chip in the series when the instrument touch screen displays the Analyzing Chip screen.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
After thermal cycling, the QuantStudio 3D Digital PCR 20K Chips can remain on the sample block for up to 24 hours. Read the QuantStudio 3D Digital PCR 20K Chips within 1 hour after you remove them from the GeneAmp 9700 PCR System sample block.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Using the Chip Carrier on the GeneAmp 9700 Dual Flat Block, up to 24 QuantStudio 3D Digital Chips can be run at one time. Please refer to Chapter 3 in the QuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) for more details on loading the chips for thermal cycling.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Yes. Never handle QuantStudio 3D Digital PCR 20K Chips or Chip Case Lids without gloves. Oils from your hands can contaminate the components and interfere with thermal cycling and imaging. For more instructions, please see the guidelines in Chapter 2 in the QuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) on loading the digital chips.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Yes, The GeneAmp 9700 PCR System must be running firmware version 3.12 or later to thermal-cycle QuantStudio 3D Digital PCR 20K Chips. The firmware version of your GeneAmp 9700 PCR System is shown on the main display after you first turn on the instrument. If needed, download and upgrade the firmware.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The system is meant to be used with the QuantStudio 3D Digital Master Mix only.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The QuantStudio 3D instrument only performs the imaging and primary analysis of the digital chips. The chips themselves must be cycled offline on a Dual Flat Block GeneAmp 9700 PCR System.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The QuantStudio 3D Digital PCR Chips are meant to be used on a Dual Flat Block Proflex or GeneAmp 9700 PCR System.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The UV glue is usable for 3 months after first opening, when stored properly. Keep it stored in an amber bag while using it on the bench, and in an amber bag in a dark place between uses.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The Tilt Base inclines the GeneAmp 9700 PCR System sample block by 11 degrees C, the angle required for optimal performance of the QuantStudio 3D Digital PCR 20K Chips.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Use all of the immersion fluid within 60 minutes of uncapping the syringe. Once a syringe is opened, you cannot reattach the cap for later use.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The QuantStudio 3D Digital PCR System features a filter set that is optimized for the FAM, VIC, and ROX dyes available from Life Technologies.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The QuantStudio 3D Digital PCR 20K Chip is a 10 mm2 high-density reaction plate that consists of a single array of 20,000 reaction wells, where each well can accommodate an 865 pL reaction. Hydrophobic coatings on the chip surface enable loading and isolation of independent PCR reactions within the reaction wells. Please see an image of the chip in the QuantStudio 3D Digital PCR System User Guide, found here:
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0007720.pdf
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
For instrument specifications, please see Appendix D, “Specifications and Layout”, in the QuantStudio 3D Digital PCR System User Guide for more details at http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Digital PCR can be used to perform rare allele detection, absolute quantitation of gene expression, viral titering, and absolute quantitation of next-generation sequencing libraries.
The QuantStudio 12K Flex system with OpenArray block and the QuantStudio 3D system are designed for digital PCR applications.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Yes, there is an option to export the Chip data as .xml on the Export tab in AnalysisSuite software v3.0.
The Poisson Plus algorithm corrects for well-to-well load volume variation on a per Chip basis. This becomes important at higher target concentrations.
You should use the Poisson Plus algorithm for projects that contain QuantStudio 3D Chips with target quantities >2,000 copies/µL.
Yes, please use Cat. No. A27322.
At low concentrations, there is no impact on the data. At higher concentrations (>2,000 copies/µL), there is a slight impact. Using the Poisson Plus algorithm will correct for well-to-well volume variation.
No significant difference has been measured.
Yes, internal studies have shown comparable performance between v1 and v2 Chips.
Yes, Firmware v3.0 will work with all versions of Chips and Lids.
No. No matter which version of Chip Lid is being used, v2 Chips require Firmware v3.0 for proper data analysis.
We recommend that you upgrade the firmware to v3.0 in order to read the 2D barcode; otherwise the .eds files will not have a Chip ID.
No, the v2 Master Mix can only be used with the v2 Chips.
No, the current v1 Master Mix can only be used with the older Chips.
Yes, the current Chips will work with the new v2 Chip Lids.
Yes, the current Lids will work on the new v2 Chips.