Pierce™ Protein A Spin Plate for IgG Screening - FAQs

No. Neither Protein A nor Protein G binds IgY. Thermo Scientific Thiophilic Adsorbent (Cat. Nos. 20500 and 44916) purifies IgY from serum. The Thermo Scientific Chicken IgY Purification Kits (Cat. Nos. 44918 and 44922) purify IgY from yolks.

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Yes. To regenerate the resins, wash wells three times with elution buffer and three times with 0.02% sodium azide in ultrapure water as detailed in the instructions.

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No. The Protein A or Protein G 96-well Spin Plates have bottom and top seals. Removing the seals will cause the buffers to leak into the wash/collection plate. It is not possible to remove only a portion of the seal and continue with the purification process, and therefore, process 96 samples each time.

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The Protein A Spin Plate binds 0.6 mg of human or 0.3-0.4 mg of mouse IgG/well (based on > 12 mg of human IgG or 6-8 mg of mouse IgG/mL of settled resin). The Protein G Spin Plate will bind > 0.5 mg human IgG/well (based on 11-15 mg of human IgG/mL of settled resin).

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Protein A and Protein G bind almost exclusively with the IgG class of antibodies, but their binding properties differ among species and subclasses of IgG. Protein A is generally preferred for rabbit, pig, dog and cat IgG. Protein G has better binding capacity for a broader range of mouse and human IgG subclasses (IgG1, IgG2, etc.), and it binds rat IgG2a and IgG2b, which do not bind to Protein A. For a comprehensive list of the binding characteristics of Protein A and Protein G, see Tech Tip #34 (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0034-Ab-binding-proteins.pdf).

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Each product includes one 96-well Spin Plate and three Wash/Collection plates (buffers are not included). Each of the 96 wells contains 50 µl of agarose resin (200 µl of 25% slurry) with the immobilized protein and can process 10-100 µl of sample.

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Protein A (Cat. No. 45202) and Protein G (Cat. No. 45204) Spin Plates for IgG Screening are for rapid, small scale, affinity-purification of polyclonal and monoclonal antibodies from serum, ascites and culture supernatants.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.