Thermo Scientific™

Pierce™ Saturated Ammonium Sulfate Solution

Catalog number:  45216

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Thermo Scientific Pierce Saturated Ammonium Sulfate Solution is formulated and ready to use for simple immunoglobulin, antibody, or other protein fractionation applications based on selective precipitation, which is also called salting out.

Features of Saturated Ammonium Sulfate Solution:

Convenient—solution is ready to use; no preparation required
Concentrates—recover antibody in 100 mL from more than 1L of cell culture supernatant
Improves purity—partial-purification of antibodies by ammonium sulfate precipitation greatly improves the final purity that can be obtained with affinity purification
Extends column life—using pre-purified antibody samples instead of crude samples enables affinity columns to be used for many more cycles
Compatible—ammonium sulfate is easily removed by dialysis or desalting columns, eliminating any obstacles to downstream methods
Versatile—use for precipitating immunoglobulins or "salting out" fractions of other sample types

The saturated ammonium sulfate solution (4.32M) enables removal of abundant proteins from serum, cell culture supernatant and ascites fluid. Slowly adding the solution to a protein sample results in decreased protein solubility and eventual protein precipitation as the charged ammonium sulfate ions compete with protein for water interaction. High molecular weight proteins, such as antibodies, are among the first to precipitate. When used in combination with antibody purification methods such as affinity chromatography, ammonium sulfate precipitation is ideal for achieving highly purified antibody samples.
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Product Line: Pierce™
Product Size: 1 L
Reagent Type: Precipitation Reagent

Contents & storage

Upon receipt store at ambient temperature.


Manuals & protocols