Thermo Scientific™

Pierce™ Protein A/G Coated Plate IP Kit

Catalog number: 45350
Thermo Scientific™

Pierce™ Protein A/G Coated Plate IP Kit

Catalog number: 45350
Catalog Number
45350
Unit Size
2 plate
Price (USD)
Price: 462.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
453502 plate
Price: 462.00
Your price:
-
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The Thermo Scientific Pierce Protein A/G Coated Plate IP Kit uses coated Protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

Pierce Coated Plate IP Kits enable rapid immunoprecipitation of multiple samples without the usual tedium of pipetting, centrifuging and separating beaded affinity resin in individual microcentrifuge tubes. Immunoprecipitation is accomplished using coated 96-well microplates rather than beaded agarose resin. The plate format allows for faster processing of multiple samples.

Features of the Protein A/G Coated Plate IP Kit:

• Ready-to-use, high quality coated plates provide high capacity and consistency
• Plate format best suited for simultaneously processing multiple samples and their control conditions
• Faster, easier and more thorough washing than with traditional tube/resin IP methods
• Uses familiar and convenient ELISA tools (multichannel pipettors and plate washing); no tedious separation of supernatant from pelleted resin beads, and no tubes to open and close and centrifuge
• Coated plates are 96-well strip plates, convenient for experiments requiring only a partial plate
• Easy-to-follow instructions, including detailed explanation of appropriate controls

Applications:
• Rapid, trouble-free immunoprecipitation of multiple samples and their controls
• Immunoprecipitation when plate-based tools and techniques are preferred

Protein A and Protein G are different proteins that bind to immunoglobulins (primarily only IgG). Typically, Protein A is preferred for use with Rabbit polyclonal antibodies, while Protein G is preferred for use with mouse antibodies (especially monoclonals of the IgG1 subclass). Protein A/G is a recombinant of Protein A and Protein G that has the additive binding properties of both proteins. Compare Protein A, Protein G and other antibody-binding proteins.

Related Products
Pierce™ Streptavidin Coated Plate IP Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
Pierce Protein A/G Coated Plate IP Kit
Assay
Affinity Purification
For Use With (Equipment)
Microplate Mixer
Product Line
Pierce™
Quantity
1 Kit
For Use With (Application)
Immunoprecipitation (IP)
Target
Not Target-Specific
Type
Immunoprecipitation/Co-Immunoprecipitation Kit
Sufficient For
192 Samples
Format
Kit

Contents & Storage

Sufficient For: Assaying 48 samples with controls or 192 total IP reactions
• Protein A/G Coated 96-well Strip Plates, 2 plates
• Uncoated 96-well Strip Plate (white), 2 plates
• PBS Pack (makes 500 mL), 1 pack
• Surfact-Amps X-100 Detergent Solution, 6 x 10 mL
• Elution Buffer (pH 2.8), 50 mL
• Neutralization Buffer, 12 mL
• Sealing Tape for 96-well Plates, 18 sheets

Store at 4°C.

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