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Invitrogen™

T4 DNA Ligase Buffer

Catalog number: 46300018
Invitrogen™

T4 DNA Ligase Buffer

Catalog number: 46300018
Catalog Number
46300018
also known as 46300-018
Unit Size
2 x 1 mL
Price (USD)
Price: 67.25
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
46300018
also known as 46300-018
2 x 1 mL
Price: 67.25
Your price:
-
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T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates for this enzyme. A T4 DNA Ligase Technical Bulletin is available.

Applications: Cloning (blunt-end or cohesive-end ligation) (2). Adding linkers or adapters to blunt-ended DNA (2).

Source: Purified from E. coli œ lysogen NM989.

Performance and Quality Testing: Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested.

Unit Definition: One unit catalyzes the exchange of 1 nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. (One unit is equal to approximately 300 cohesive-end ligation units.)

Unit Reaction Conditions: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2 , 10 mM DTT, 66 μM ATP, 3.3 μM 32 P-labeled pyrophosphate, and enzyme in 0.1 ml for 20 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition
Approved for shipment on Wet or Dry Ice
Compatible Buffer
5X Reaction Buffer
Quantity
2 x 1 mL
Product Type
T4 DNA Ligase Buffer

Contents & Storage

T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2 , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.

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