DyLight™ 530-R2 NHS Ester

Catalog number: 46611

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Label peptides, antibodies and other proteins at primary amines with the rhodamine-based, water-soluble Thermo Scientific DyLight Green-emitting Specialty Dye, which is comparable to Alexa Fluor 532.

DyLight Green-emitting Dye is a labeling agent that provides bright fluorescence detection for imaging. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetration in vivo, while more hydrophilic dyes have less nonspecific binding potential. This dye contains 2 sulfonates for increased solubility and an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.

Features of DyLight 530-R2 NHS Ester:

NHS ester reactive group—allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
Water solubility—2 sulfonate groups increase the solubility of this dye over over less-sulfonated dyes

Applications:
• Imaging
• Antibody labeling
• Direct immunofluorescence staining
• Flow cytometry
• Fluorescence correlation spectroscopy
• ELISA
• Western blotting
• Protein microarrays
• Polymer labeling
• Peptide labeling
• Phalloidin labeling for actin staining
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: DyLight™ Fluor (Specialty)
Chemical Reactivity: Amine
Solubility: DMF (Dimethylformamide)
Product Size: 1 mg
Excitation⁄Emission (nm): 533⁄554

Contents & storage

Store desiccated at -20°C. Shipped at ambient temperature.

Documents

Manuals & protocols