Los primers aleatorios son oligodesoxirribonucleótidos (en su mayoría hexámeros) que se utilizan para preparar sondas de ADN etiquetado a partirMás información
Have Questions?
Número de catálogo
Cantidad
48190011
100 μl
Número de catálogo 48190011
Precio (USD)
155,40
Each
Añadir al carro de la compra
Cantidad:
100 μl
Precio (USD)
155,40
Each
Añadir al carro de la compra
Los primers aleatorios son oligodesoxirribonucleótidos (en su mayoría hexámeros) que se utilizan para preparar sondas de ADN etiquetado a partir de plantillas para la hibridación mediante filtro y la hibridación in situ y para cebar ARN mensajero (ARNm) con o sin poli(A) para la síntesis de ADNc. Estos primers son realmente aleatorios y adecuados para la síntesis de ADN mediante fragmentos Klenow con plantillas de ADN. También se destinan a la síntesis de ADNc mediante transcriptasa inversa con plantillas de ARNm. Para evitar interferencias del tampón de almacenamiento, se suministran primers aleatorios en un tampón con bajas concentraciones de sal.
Pruebas de rendimiento y calidad: La incorporación de un nucleótido etiquetado radiactivamente se verifica mediante el ADN de control en una reacción de etiquetado de primers aleatorios.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Para utilizar con (aplicación)Síntesis de ADNc
FormularioLíquido
Tipo de productoPrimer
Cantidad100 μl
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Concentración3 μg/μL
PrimerAleatorio
Unit SizeEach
Contenido y almacenamiento
• Cebadores aleatorios en Tris-HCl 3 mM, EDTA 0.2 mM (100μ L a las 3μ g/μ L)
Conservar en– 20° C. Estabilidad garantizada durante 6 meses si se almacena correctamente.
Preguntas frecuentes
Can I purchase Random Primers separately?
Yes, we do offer Random Primers as a stand-alone item: Cat. No. 48190011.
Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?
The following components are available as stand-alone items:
- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)
What is the concentration of Random Primers (Cat. No. 48190011)?
The concentration of Random Primers (Cat. No. 48190011) is 1500 pmol/µL or 1.5 mM.
Global Expression Profiling of Acetate-grown Escherichia coli.
Authors: Oh Min-Kyu; Rohlin Lars; Kao Katy C; Liao James C;
Journal:J Biol Chem
PubMed ID:11815613
'This study characterized the transcript profile of Escherichia coli in acetate cultures using DNA microarray on glass slides. Glucose-grown cultures were used as a reference. At the 95% confidence level, 354 genes were up-regulated in acetate, while 370 genes were down-regulated compared with the glucose-grown culture. Generally, more metabolic genes ... More
RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs.
Authors:Ebinu JO, Bottorff DA, Chan EY, Stang SL, Dunn RJ, Stone JC
Journal:Science
PubMed ID:9582122
RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of EF hands that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling ... More
Class II histone deacetylases act as signal-responsive repressors of cardiac hypertrophy.
Authors:Zhang CL, McKinsey TA, Chang S, Antos CL, Hill JA, Olson EN,
Journal:Cell
PubMed ID:12202037
The heart responds to stress signals by hypertrophic growth, which is accompanied by activation of the MEF2 transcription factor and reprogramming of cardiac gene expression. We show here that class II histone deacetylases (HDACs), which repress MEF2 activity, are substrates for a stress-responsive kinase specific for conserved serines that regulate ... More
Improvements in siRNA properties mediated by 2'-deoxy-2'-fluoro-beta-D-arabinonucleic acid (FANA).
Authors:Dowler T, Bergeron D, Tedeschi AL, Paquet L, Ferrari N, Damha MJ,
Journal:Nucleic Acids Res
PubMed ID:16554553
RNA interference (RNAi) has emerged recently as an efficient mechanism for specific gene silencing. Short double-stranded small interfering RNAs (siRNAs) are now widely used for cellular or drug target validation; however, their use for silencing clinically relevant genes in a therapeutic setting remains problematic because of their unfavourable metabolic stability ... More
Use of an in vivo reporter assay to test for transcriptional and translational fidelity in yeast.
Authors: Shaw Randal J; Bonawitz Nicholas D; Reines Daniel;
Journal:J Biol Chem
PubMed ID:12006589
Eukaryotic RNA polymerase II and Escherichia coli RNA polymerase possess an intrinsic ribonuclease activity that is stimulated by the polymerase-binding proteins SII and GreB, respectively. This factor-activated hydrolysis of nascent RNA has been postulated to be involved in transcription elongation as well as removal of incorrect bases misincorporated into RNA. ... More