p-Bromophenacylate Reagent (p-bromophenacyl bromide) (0.1 mmol/mL)

Catalog number: 48891

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Thermo Scientific Pierce p-Bromophenacylate Reagent (formerly Phenacyl-8) reacts with carboxylic acids to provide quantitative detection of peptides and amino acids with few or no side reactions.

Bromophenacyl bromide is an excellent derivatization reagent for analysis of carboxylic acids. The reagent is useful for preparation of phenacyl esters, which are used to separate many saturated and unsaturated fatty acids for UV detection in HPLC applications with low nanomole sensitivity. The compound is also useful for studying acid composition of bacterial cell walls. Our p-Bromophenacylate Reagent is a ready-to-use solution of the chemical with crown ether in acetonitrile.

Features of p-Bromophenacylate Reagent:

• Ready-to-use reagent solution eliminates the need to premix of phenacylbromide and crown ether
• Derivatization is rapid and quantitative, with yields greater than 95% in 15 to 20 minutes at 80°C
• Excess reactants do not interfere with detection
• Large excess of alkylating reagent is not necessary
• Small amounts of water or alcohol do not interfere
• If isolation is desired, products are usually crystalline

Reagent Properties:

• Formulation: 0.1mmol/mL p-bromophenacyl bromide and 0.005mmol/mL crown ether in acetonitrile
• Form: Colorless to light yellow liquid
• Reactive toward: Carboxylic acids (—COOH)
• Detection: UV absorption (λmax = 260nm)
• Application: Precolumn derivatization for nanomole levels of detection by HPLC
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 10 mL
Chemical Reactivity: Carboxylic Acid

Contents & storage

Store at room temperature.

Documents

Manuals & protocols