Thermo Scientific™

FDAA (Marfey's Reagent) (1-fluoro-2-4-dinitrophenyl-5-L-alanine amide)

Catalog number:  48895

 Related applications:

Protein Assays and Analysis

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Thermo Scientific Pierce Marfey's Reagent (FDAA) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.

Features of Marfey's Reagent (FDAA):

• For precolumn derivatization of primary amines for detection by UV absorbance (λmax = 340nm)
• Provides nanomole sensitivity in typical HPLC applications with amino acids
• Reaction products enable chiral separation of D- and L-amino acids
• Following reverse-phase HPLC, derivatives have an extinction coefficient of 3 x 10^4M-1 cm-1

FDAA is 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide, also called Marfey's reagent. The compound reacts with primary amines and is used as a derivatization reagent for UV detection in liquid chromatography methods. The reagent provides for detection at 340nm with nanomole sensitivity. FDAA derivatives of D-amino acids exhibit strong intramolecular bonding, which reduces their polarity relative to the corresponding L-amino acid derivatives. Consequently, the D-derivatives are selectively retained on reverse phase columns and elute much later than corresponding L-derivatives.

Reagent Properties:
• Alternative names: FDAA, Marfey's Reagent
• Chemical name: 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide; N2-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide
• Reactive toward: Primary amines (—NH2)
• Chemical formula: C9H9FN4O5
• CAS number: 95713-52-3
• Molecular weight: 272.19
• Form: Bright yellow or orange crystals or powder, free of particulate matter

FDAA derivatives can be separated with simple linear gradient of triethylamine phosphate/acetonitrile on a Spheri-5 RP-18 HPLC cartridge column. FDAA reduces the need for a chiral column because the D-derivatives are retained on the column, eluting after the L-derivatives. The derivatization is easy to perform and completed in 90 minutes, resulting in derivatives that are stable for at least 48 hours.
For Research Use Only. Not for use in diagnostic procedures.


Chemical Reactivity: Amine
Product Size: 50 mg

Contents & storage

Upon receipt store product at room temperature.


Manuals & protocols