Thermo Scientific™

CaptureSelect™ C-tagXL Pre-packed Column

Catalog number:  494307201

Related applications:

Chromatography

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Description

CaptureSelect C-tagXL Affinity Matrix is an improved version of CaptureSelect C-tag Affinity Matrix with a significantly increased binding capacity, offered here in pre-packed format.

Like C-tag Affinity Matrix, C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.

The affinity matrix is offered in a pre-packed format as a set of 5 x 1 mL columns or as one 5 mL column, with connectors that are compatible with standard chromatography equipment.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Bottle(s)
Immobilized Phase: Anti C-tagXL
Product Line: CaptureSelect™
Product Size: 5 x 1 mL
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C).