CaptureSelect™ C-tagXL Pre-packed Column

CaptureSelect C-tagXL Affinity Matrix is an improved version of CaptureSelect C-tag Affinity Matrix with a significantly increased binding capacity, offered here in pre-packed format.

Like C-tag Affinity Matrix, C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.

The affinity matrix is offered in a pre-packed format as a set of 5 x 1 mL columns, or as one 5 mL column, with connectors that are compatible with standard chromatography equipment.