CaptureSelect™ Pre-packed Column KappaXL

Catalog number: 494321001

 Related applications: Chromatography

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CaptureSelect™ KappaXL Affinity Matrix, available here in a pre-packed column format, has been specifically designed for the purification of recombinant human Ig’s (including IgA, IgM, IgD, and IgE) containing a Kappa light chain and their Fab fragments.

Features of this affinity matrix include:
• Mild elution for Fab fragments and antibodies
• Human specific (e.g., no binding to bovine antibodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect™ KappaXL Affinity Matrix purifies Ig’s, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.

Free of animal components
CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single-domain antibody fragments. The ligand is a 13 kDa single-domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.


Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ KappaXL affinity ligand
Coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~30 g IgG/L resin at 5-10 minutes contact time
Elution conditions: 20 mM citric acid, pH 3.5
Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.


Format: Chromatography Cartridge
Column Type: Affinity Column
Product Size: 5 x 1 mL columns
Immobilized Phase: Anti-Kappa light chain
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols